主要组织相容性复合体在人乳腺癌组织的表达

The Expression of Major Histocompatibility Complex in Human Breast Cancer Tissue

目的通过观察主要组织相容性复合体(MHC)在乳腺癌组织的表达,探讨MHC与乳腺癌发生发展的关系,为临床进行乳腺癌的生物治疗提供实验依据。方法收集手术切除的人乳腺癌组织和癌旁相对正常乳腺组织,用免疫组织化学方法和图像分析技术,观察人白细胞抗原(HLA-DR)阳性细胞、CD4+T细胞和TCD8+T细胞在不同乳腺组织中的表达。结果在乳腺癌组织中HLA-DR阳性细胞平均光密度值和面数密度明显高于正常乳腺组织(P<0.05);CD4+T和TCD8+T细胞阳性细胞面数密度与正常乳腺组织相比明显减少(P<0.05),且CD4+T细胞多分布在HLA-DR阳性细胞附近。结论人乳腺癌局部微环境中HLA-DR阳性细胞数多于正常乳腺组织,CD4+T和TCD8+T在人乳腺癌组织低于乳腺正常组织。

Objective To investigate the expression of major histocompatibility complex （MHC） positive cells in human breast cancer tissues to provide the experimental data for the further biological therapy of breast cancer. Methods The human breast cancer tissue and normal breast tissue were collected and the expression of HLA-DR, CD4 and CD8 were observed with immunohistologieal staining and analyzed with mieroimge analysis system. Results The mean optic density and square density of HLA-DR positve cells were increased in breast cancer tissue than in normal breast tissue （ P 〈0.05 ）. The square density of CD4^＋T and CD8^＋ T positive cells in breast cancer tissue was obviously lower than that in normal breast tissue. Conclusion MHC was overexpressed and CD4^＋T and CD8^＋T were downexpressed in local micro-environment of breast cancer.