变应性鼻炎患者外周血T-BET的表达及其与IGE和嗜酸粒细胞阳离子蛋白的关系

Expression of T-bet and its relation with IgE and eosinophile cationic protein in patients with allergic rhinitis

目的探讨变应性鼻炎(ALLERGIC RHINITIS,AR)患者外周血单核细胞(PERIPHERAL BLOODMONOUCLEAR CELLS,PBMC)中转录因子T-BET MRNA的表达及其与血清总IGE(TIGE)、患者鼻过敏症状和嗜酸粒细胞阳离子蛋白(EOSINOPHILE CATIONIC PROTEIN,ECP)的关系。方法用UNICAP CAP系统检测血清变应原、TIGE和ECP的含量。取22例对尘螨过敏的AR患者和8例健康人静脉血,密度梯度离心分离PBMC,其中部分PBMC用50ΜG/ML螨变应原培养刺激,用逆转录-聚合酶链反应(REVERSETRANSCRIPTASE-POLYMERASE CHAIN REACTION,RT-PCR)检测T-BET MRNA的表达。结果①AR患者和正常人T-BET MRNA表达强度与参照物Β-ACTIN的比率(X-±S)分别是0·381±0·099和0·750±0·067,差异有统计学意义(P<0·01);②T-BET MRNA的表达强度分别与AR患者症状评分、ECP的水平没有相关性(R分别是0·187和-0·165,P值均>0·05);③而T-BET MRNA表达强度与患者血清TIGE水平呈负相关(R=-0·525,P<0·05);④变应原刺激前后T-BET MRNA表达强度分别是0·381±0·099和0·365±0·104,差异无统计学意义(P>0·05)。结论变应原为螨的AR患者PBMC中,T-BET MRNA表达较健康人低,T-BET MRNA低表达是AR发生TH1和TH2失衡机制中的重要环节之一。T-BET MRNA的下调表达程度与AR患者症状严重程度和ECP浓度无关。特异性变应原的刺激对AR患者PBMC中的T-BET表达没有影响。T-BET是间接影响IGE水平的因素之一。

Objective To investigate the expression of T-bet mRNA in peripheral blood monouclear cells （PBMC） as well as its relations with total IgE （TIgE）, eosinophile cationic protein （ECP） in serum and nasal allergic symptoms in patients with allergic rhinitis （AR）. Methods The allergen, TIgE and ECP in serum of patients with AR were detected by Unicap CAP system. Blood sample was taken from 8 healthy individuals and 22 patients with allergic rhinitis. PBMC was isolated by density gradient centrifugation and one part of them was cultured with 50 μg/ml mite allergen. PBMC was subjected to analysis of T-bet mRNA expression using semiquantitative reverse transcriptase-pelymerase chain reaction （RT-PCR）. Results The ratio of T-bet to β-actin mRNA levels was 0. 381 ± 0. 099 in patients and 0. 750 ±0. 067 in normal individuals, the difference was significantly （ P 〈 0. 01 ）. The expression intensity of T-bet mRNA had no relation to varying severity of allergic symptoms and concentration of ECP and the correlation coefficient was 0. 187 and -0. 165 （all P 〉 0.05）. However, there was an inverse correlation between expression intensity of T-bet mRNA and TIgE concentration （ r = - 0. 525, P 〈 0. 05 ）. Mean mRNA level （^-x ＋ s） of T-bet expression before and after being stimulated by allergen was 0. 381 ± 0. 099 and 0. 365 ± 0. 104 respectively, which indicated no significant differences （ P 〉 0. 05 ）. Conclusions Among allergic patients whose allergen was mite, there was a down-regulated expression of T-bet mRNA, which had no relation to ECP concentration and allergic symptoms, but was one of important links in mechanisms of imbalance of Th1/Th2 in AR. There was no effect of specific allergen on T-bet mRNA in patients with AR. T-bet was one of indirect factors that affected the level of IgE.