摘要
目的观察哇巴因(Ouabain)对血管紧张素Ⅱ(AngⅡ)及其1型(AT1)与2型(AT2)受体mRNA在心肌中表达的影响。方法60只SD大鼠随机分为3组,分别为Ouabain组[27.8 ng/(kg.d)Ouabain腹腔注射]、Losartan组[27.8 ng/(kg.d)Ouabain腹腔注射,同时给予Losartan 30 ng/(kg.d)灌胃]、对照组(生理盐水2 mL/d腹腔注射),每周测定鼠尾血压,共6周。应用放免法测定各组大鼠血浆及心肌中AngⅡ质量浓度,同时采用实时定量荧光PCR(FQ-PCR)观察心肌中AT1和AT2mRNA表达的变化。结果血浆中的AngⅡ质量浓度在Ouabain组及Losartan组均显著高于对照组(P<0.05),而Ouabain组心肌中AngⅡ质量浓度无明显变化。与对照组相比,Ouabain组及Losartan组心肌中AT1mRNA与AT2mRNA的表达明显上调(P<0.05)。结论外周长期、慢性给予Ouabain后可持续升高SD大鼠的血压,并使RAS系统激活,这一作用可能是通过AT1受体介导。给予Ouabain激活AT1受体的同时,AT2活性也增加。
Objective To receptors in the myocardium of investigate how ouabain influence angiotensin Ⅱ(Ang Ⅱ ) and its subtype 1 and 2 ouabain-induced hypertensive rats. Methods 60 Sprague-Dawly (SD) rats were randomly divided into three groups: two experimental groups and a control group. One of the experimental groups was injected with 27.8 ng/(kg · d) ouabain intraperitoneally, the other was injected with 27.8 ng/(kg· d) ouabain and losartan 30 ng/(kg ·d). The control group was given placebo. Then, the blood pressure (BP) were measured every week and all rats were killed 6 weeks later. Radioimmunity and FQ-PCR were used to observe the changes of Ang Ⅱ and its subtype 1 and 2 receptors in the myocardium of these ouabain-induced hypertension rats. Results Plasma Ang Ⅱ of the ouabain-induced hypertension group was higher than that of the control group ( P〈0.05), but these was no change in the myocardium Ang Ⅱ . AT1 rnRNA and AT2 mRNA in the ouabain-induced hypertension group was remarkablely higher than that in the control group (P〈0.05). Conclusion A long-term and peripheral administration of ouabain can raise blood pressure of rats, which may be induced by AT1 receptor. Ouabain can activate AT1 receptor as well as AT2 receptor.
出处
《西安交通大学学报(医学版)》
CAS
CSCD
北大核心
2005年第6期526-529,共4页
Journal of Xi’an Jiaotong University(Medical Sciences)