有机磷农药混剂对大鼠的胚胎毒性和致畸性研究

Study on the teratogenesis and embryo toxicity of mixture of organophosphorus pesticide in rats

目的观察敌敌畏、乐果和马拉硫磷对大鼠的致畸作用。方法将SD孕鼠随机分为4组,即有机磷农药混剂20.4、30.64、0.8 mg/kg 3个剂量染毒组和阴性对照组。分别经口给予不同剂量的有机磷农药混剂和生理盐水,进行传统的致畸实验,观察母鼠及胎仔的情况。结果有机磷农药混剂对母鼠体质量有影响,40.8 mg/kg组体质量明显减轻。孕鼠的生殖能力包括平均黄体数、胎盘重和窝重与阴性对照组比较也有显著性差异(P<0.05)。染毒组胎仔体质量与阴性对照组比较有显著性差异(P<0.05)。40.8 mg/kg和30.6 mg/kg组还出现死胎、吸收胎和畸胎,但与阴性对照组比较无显著性差异(P>0.05)。结论在实验剂量范围内,有机磷农药混剂对大鼠有母体和胚胎毒性。

Objective To observe the potentially teratogenic effects of the mixture of organphosphorus （ops） in SD rats. Methods SD pregnant rats were randomly divided into four groups （20. 4, 30. 6, 40. 8 mg/kg treatment groups and negative control）. Conventional teratogenicity test was performed. Pregnant rats in the treatment groups and control group orally received different concentration of mixture of organophosphorus pesticide and saline on days 6-15 of gestation, respectively. At the same time, the status of pregnant rats and fetuses were observed. Results Changes of weight in 30.6 and 40.8 mg/kg trcatmcnt groups were observed. Compared with negative control, all indexes of procreative ability of prcgnant rats in treatment groups had significant differences with those in negative control （P〈0.05）; embryo weights of the ops compound had decreased in all treatment groups; there were dead fetuses, absorbent fetuses and malformed fetuses in 30.6 and 40.8 mg/kg groups, but there were no significant differences with negative control. Conclusion Within the range of experiment doses, the ops compound is considered to have maternal, embryo toxicity and teratogenicity in SD rats.