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同种异体大鼠角膜移植物TRAIL及其受体DR4的表达与免疫排斥反应的关系 被引量：2

Expression of TRAIL and DR4 in rejected rat corneal allografts and the relationship between them and immune rejection
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摘要 目的观察大鼠角膜移植术后角膜组织中肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis-inducingligand,TRAIL)及其死亡受体4(death receptor4,DR4)的表达情况,分析其与角膜移植免疫排斥反应的关系。方法实验对象分为移植组和对照组,移植组采用大鼠同种异体穿透性角膜移植模型,术后每日观察排斥反应指数的变化,分别于术后不同时间点(7d、10d、14d)取角膜植片,采用免疫组织化学法(SABC法)检测TRAIL及DR4蛋白表达情况,并与对照组正常大鼠角膜组织TRAIL及DR4蛋白表达情况相比较。结果TRAIL及DR4主要表达于正常角膜上皮层,在基质层及内皮层有极少量表达;而移植组急性排斥期角膜植片各层TRAIL及DR4表达均增高,阳性表达主要集中在植片伤口附近的上皮层及浅层基质。结论与正常角膜相比,同种异体角膜移植术后急性排斥期植片各层TRAIL及DR4蛋白表达均增高,由此推测TRAIL及其受体DR4与角膜移植急性免疫排斥反应的发生发展有关。 Objective To investigate the expression of TNF-related apoptosis-inducing ligand（TRAIL）and its receptor DR4 in rejected rat corneal allografts and the relationship between them and immune rejection. Methods Corneal allograft transplantation were performed orthotopically in Wistar rats and SD rats（Wistar→SD）. Corneal samples were collected on day 7,10 and 14 postoperatively. Immunohistochemistry was used to detect the expression and distribution of TRAIL/DR4 protein in corneal grafts during allograft rejection. The corneas of normal Wistar rats were set as control. Results TRAIL and its receptor DR4 were detected on normal cornea, it was mainly expressed on the epithelium, with modest staining in the stroma and endothelium. The expression of TRAIL and its receptor DR4 were increased significantly during allograft rejection in the epithelium, endothelium and stroma of corneal allografts. Conclusion The expression of TRAIL and DR4 are increased significantly during allograft rejection in the corneal allografts compared with the normal corneas. TRAIL and its receptor DR4 may be related to the acute immune rejection after penetrating keratoplasty.
作者 袁伟 陆晓和 张永强 贺玲 宫玉波 彭小娟
机构地区 南方医科大学珠江医院眼科 解放军第
出处 《眼科新进展》 CAS 2005年第6期495-497,共3页 Recent Advances in Ophthalmology
基金 广东省自然科学基金资助(编号:032839)~~
关键词 穿透性角膜移植 肿瘤坏死因子相关凋亡诱导配体 死亡受体4 免疫组织化 keratoplasty penetrating TNF-related apoptosis-inducing ligand death receptor 4 immunohistochemistry
分类号 R772.2 [医药卫生—眼科]
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参考文献10

  • 1Wiley SR,Schooley K,Smolak PJ,Din WS,Huang CP,Nicholl JK,et al.Identification and characterization of a new member of the TNF family that induces apoptosis [J].Immunity 1995;3(6) :673-682.
  • 2Pitti RM,Marsters SA,Ruppert S,Donahue CJ,Moore A,Ashkenazi A,et al.Induction of apoptoois by Apo2 ligand,a new member of the tumor nectosis factor cytokine family[J].J Bio1 Chem 1996;271(22):12687-12690.
  • 3Williams KA,Coster DJ.Penetrating corneal transplantation in theinbred rat:a new model[ J].Invest Ophthalmol Vis Sci 1985;26(1):23-30.
  • 4Holland EJ,Chan CC,Wetzig RP,Palestine AG,Nussenblatt RB.Clinical and immunohistoogic studies of corneal rejection in the rat penetrating keratoplasty model [ J ].Cornea 1991; 10(5) :374-380.
  • 5Jeremias I,Herr I,Boehler T,Debatin KM.TRAIL/Apo-2-ligand-induced apoptosis in humsn T cells[J].Eur JImmunol 1998; 28(1):143-152.
  • 6Pan G,O'Rourke K,Chinnaiyan AM,Gentz R,Ebner R,Ni J,et al.The receptor for the cytotoxic ligand TRAIL[ J].Science 1997;276(5309):111-113.
  • 7张永强,陆晓和,徐宁.细胞凋亡与角膜移植免疫[J].眼科新进展,2002,22(5):358-360. 被引量：6
  • 8Song CJ,Liu XS,Zhu Y,Chen LH,Jia W,Li YN,et al.Expression of TRAIL,DR4,and DR5 in kidney and serum from patients receiving renal transplantation[J].Transplant Proc 2004;36(5):1340-1343.
  • 9Xie L,Shi W,Guo P.Roles of tumor necrosis factor-related apoptosis-inducing ligand in corneal transplantation [J].Transplantation 2003;76(11):1556-1559.
  • 10Song K,Chen Y,Goke R,Wilmen A,Seidel C,Goke A,et al.Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an inhibitor of autoimmune inflammation and cell cycle progression[ J].Exp Med 2000; 191 (7):1095-1104.

二级参考文献22

  • 1[1]Williams KA,Muehlberg SM,Lewis RF,Coster DJ. How successful is corneal transplantation-a report from the Australian corneal graft register[J].Eye 1995;9(pt2): 219-227.
  • 2[2]Rocha G, Deschenes J, Rowsey JJ. The immunology of corneal graft rejection[J]. Critl Rev Immunol 1998;18(4): 305-325.
  • 3[3]Bellgrau D,Gold D,Selawry H,Moore J,Franzusoff A,Duke RC. A role for CD95 ligand in preventing graft rejection[J]. Nature 1995;377(6550): 630-632.
  • 4[4]Yamagami S,Kawashima H,Tsuru T,Yamagami H,Kayagaki N,Yagita H,et al. Role of fas-fas ligand interactions in the immunorejection of allogeneic mouse corneal transplants[J]. Transplantation 1997;64(8): 1107-1111.
  • 5[5]Stuart PM,Griffith TS,Usui N,Pepose J,Yu X,Ferguson TA. CD95 ligand(FasL)-induced apoptosis is necessary for corneal allograft survival[J]. J Clin Invest 1997;99(3): 396-402.
  • 6[6]Wilson SE,Li Q,Weng J, Barry Lane PA,Jester JV, Liang Q,et al. The Fas/Fas ligand system and other modulators of apoptosis in the cornea[J]. Invest Ophthalmol Vis Sci 1996;37(8): 1582-1592.
  • 7[7]Li XY, De Marco BM, Mayhew ES, Niederkorn JY. Aqueous humor-borne factor upregulates Bcl-2 expression in corneal endothelial cells[J]. Curr Eye Res 1998;17(10):970-978.
  • 8[8]Griffith TS, Ferguson TA. The role of FasL-induced apoptosis in immune privilege[J]. Immunol Today 1997;18(5): 240-244.
  • 9[9]Krams SM, Egawa M, Quinn MB, Martinez OM. Apoptosis as a mechanism of cell death in a rat model of liver allograft rejection[J]. Transplantation Proc 1995;27(1):466-467.
  • 10[10]Suzuki I, Fink PJ. Maximal proliferation of cytotoxic T lymphocytes requires reverse signaling through Fas ligand[J]. J Exp Med 1998;187(1): 123-128.

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眼科新进展
2005年 第6期

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