海水浸泡对角膜上皮损伤后组织愈合影响

Effect of seawater soaking on the tissue healing after corneal epidermis injury

目的研究海水浸泡对角膜上皮伤后组织愈合的影响。方法对10只灰兔表麻下刮除双侧角膜缘内上皮,右眼为实验眼,左眼为对照眼。实验眼海水浸泡0.5h,对照眼生理盐水浸泡0.5h。分别于伤前和伤后1d、3d、5d、7d、10d进行角膜厚度测定、角膜上皮荧光染色、裂隙灯下检查。按期依序处死2只实验兔,取全眼球甲醛固定后,石蜡包埋切片,常规染色,光镜下观察。角膜厚度值分别进行伤前与伤后自身对照t检验,实验眼和对照眼t检验。结果角膜去上皮损伤后,裂隙灯下见伤后角膜明显水肿混浊并逐日加重,且实验眼较对照眼严重,实验眼伤后10d可见角膜缘有大量新生血管长入。角膜上皮荧光染色,伤后均为阳性,但实验眼明显大于对照眼。光学显微镜下见实验眼上皮细胞愈合明显延迟,角膜组织炎性反应明显加重,基质血管化加重。伤后实验眼角膜厚度值(0.788±0.086)mm与伤前角膜厚度值(0.417±0.029)mm经检验有显著差异(t=2.878,P<0.01)。伤后实验眼角膜厚度与对照眼角膜厚度(0.656±0.085mm)经2组均数t检验有显著差异(t=2.762,P<0.01)。结论海水浸泡对伤后上皮组织的愈合有不良影响。

Objective To study the effect of seawater soaking on the healing of cornea after explosive injury. Methods Corneal epidermis were scraped from 20 eyes of 10 adult grey rabbits. For each rabbit, the right eye was set as experimental group and the left one was contorl. After the injury, the experimental eyes were soaked in seawater for 30 minutes and the contorl eyes were soaked in 9 g·L^-1 sodium chloride injection for the same time. All eyes were underwent slit lamp examinations, fluorescein staining and ultrasonic cornea thickness metering on the corneas before the injury and 1 day,3 days,5 days,7 days, 10 days after the injury, respectively. Tow rabbits were killed according to the time mentioned above and the eyes were examined with pathology. The cornea thickness numbers were compared using self-comparison test and group t test. Results After scraping the whole corneal epidermis, slit lamp microscope examination found that the corneas were edema and turbidity; the experimental eyes were stricter than the contorl eyes. The corneas of the experimental eye were vascularized 10 days after the injury. Fluorescein staining showed that more apparent pigmentation was found on the experimental eyes than that on the contorl ones. Comparative examination under the optical microscope showed that the corneal tissue of the experiment eyes changed distinctly,and its inflammation was severe and healing was delayed and the vascularization degree became high after the injury. Cornea thickness of the experimental eyes（0. 788 mm ± 0. 086 mm）, became apparently thicker than that of the control eyes（0. 656 mm ± 0. 085 mm）. The difference was statistically signifieant（t=2.762,P〈0.01）.Conclusion Seawater soaking showed bad effects on the healing of the corneal epidermis irjury.