摘要
目的探讨雌激素对喹啉酸(QA)所致的大鼠纹状体神经元损伤的影响。方法将成年雌性大鼠切除双侧卵巢后随机分为卵巢切除组(非替代组)和卵巢切除+雌二醇组(替代组);向纹状体注射QA,采用尼克酰胺腺嘌呤二核苷酸磷酸(NADPH)黄递酶组化法检测纹状体NADPH阳性神经元数,记录阿朴吗啡诱发大鼠旋转次数,分别用黄嘌呤氧化酶法和硫代巴比妥法测定纹状体超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果与非替代组比较,替代组大鼠损伤侧纹状体NADPH阳性神经元明显增多(P<0.05),SOD活性明显增高(P<0.05),MDA含量明显降低(P<0.01),阿朴吗啡诱发的大鼠旋转次数明显减少(P<0.01)。结论雌激素可能通过减轻氧化应激反应对QA引起的纹状体神经元损害起保护作用。
Objective To explore the effect of estrogen on striatal neurons damage in rats induced by quinolinic acid (QA). Methods Ovariectomized rats were divided into two groups : non-replacement treatment group ( recievied intrastriatal application of QA alone ) and replacement treatment group ( received both QA and 17-β estradiol ). Striatal neurons injury was evaluated using NADPH-diaphorase histochemistry and the rotation number of rats induced by apomorphine was recorded. Activity of SOD and contents of MDA in striatum was measured by xanthine oxidase and thio-barbital method, respectively. Results Compared with non-replacement treatment group, there was a significant increase in the number of NADPH-diaphorase positive neurons ( P 〈 0. 05 ) , increased activity of SOD ( P 〈0.05 ), decreased contents of MDA ( P 〈 0.01 ) and reduction of rotation number induced by apomorphine (P 〈0. 01 ) in the injured side of striatum in replacement treatment group. Conclusion Estrogen may have a neuroprotective role on striatal neurons injury induced by QA through decreasing oxidative stress reaction.
出处
《临床神经病学杂志》
CAS
北大核心
2005年第6期443-445,共3页
Journal of Clinical Neurology
关键词
喹啉酸
雌激素
亨廷顿病
estrogen
quinolinic acid
Huntington's disease