雌激素对喹啉酸致大鼠纹状体神经元损害的影响

Effects of estrogen on striatal neurons damage in rats induced by quinolinic acid

目的探讨雌激素对喹啉酸(QA)所致的大鼠纹状体神经元损伤的影响。方法将成年雌性大鼠切除双侧卵巢后随机分为卵巢切除组(非替代组)和卵巢切除+雌二醇组(替代组);向纹状体注射QA,采用尼克酰胺腺嘌呤二核苷酸磷酸(NADPH)黄递酶组化法检测纹状体NADPH阳性神经元数,记录阿朴吗啡诱发大鼠旋转次数,分别用黄嘌呤氧化酶法和硫代巴比妥法测定纹状体超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果与非替代组比较,替代组大鼠损伤侧纹状体NADPH阳性神经元明显增多(P<0.05),SOD活性明显增高(P<0.05),MDA含量明显降低(P<0.01),阿朴吗啡诱发的大鼠旋转次数明显减少(P<0.01)。结论雌激素可能通过减轻氧化应激反应对QA引起的纹状体神经元损害起保护作用。

Objective To explore the effect of estrogen on striatal neurons damage in rats induced by quinolinic acid （QA）. Methods Ovariectomized rats were divided into two groups ： non-replacement treatment group （ recievied intrastriatal application of QA alone ） and replacement treatment group （ received both QA and 17-β estradiol ）. Striatal neurons injury was evaluated using NADPH-diaphorase histochemistry and the rotation number of rats induced by apomorphine was recorded. Activity of SOD and contents of MDA in striatum was measured by xanthine oxidase and thio-barbital method, respectively. Results Compared with non-replacement treatment group, there was a significant increase in the number of NADPH-diaphorase positive neurons （ P 〈 0. 05 ） , increased activity of SOD （ P 〈0.05 ）, decreased contents of MDA （ P 〈 0.01 ） and reduction of rotation number induced by apomorphine （P 〈0. 01 ） in the injured side of striatum in replacement treatment group. Conclusion Estrogen may have a neuroprotective role on striatal neurons injury induced by QA through decreasing oxidative stress reaction.