终末肝病患者血清和肝组织中乙型肝炎病毒转录体的对照研究

Contrastive study on hepatitis B virus transcript of serum and liver tissue in patients with end-stage liver disease

目的分析HBV转录体与终末肝癌肝硬化的关系。方法用血清核酸提取试剂盒及TRIzol从血清及肝组织中提取核酸,PCR及RT-PCR扩增HBV XDNAf、RNA和trRNA,琼脂糖电泳显示结果并进行分析。结果HBV DNA和转录体在HBsAg(+)血清中的检出率显著高于HBsAg(-)中的检出率(P<0.01);fRNA在HBsAg(+)/HBeAg(+)血清中的检出率明显高于HBeAg(-)中的检出率(P<0.01)。trRNA在HBsAg(-)的隐匿性感染患者血清中有高检出率44.0%,且同一患者血清和组织中同时检出的一致率高。结论HBV转录体与终末期肝病的发生有密切的关系。trRNA具备作为诊断HBV感染新的血清学标志物的潜在可能。

Objective To analyse the relationship between HBV transcript and end-stage liver disease. Methods Nucleic acid was distilled from serum and liver tissue with High pure viral nucleic acid kit and TRIzol reagent. Hepatitis B virus （HBV） X region deoxyribonucleic acid （DNA）, full-length RNA （fRNA） and truncated RNA （trRNA） were amplified by polymerase chain reaction （PCR） and Reverse transcriptase-polymerase chain reaction （RT-PCR）. Their products were displayed by agarose electrophoresis and result were analysed. Results HBVxDNA and transcript were detected higher in HBsAg （ ＋ ） serum than that in HBsAg（ - ） serum （p 〈 0.01）. tRNA detected in HBsAg（ ＋ ）/HBeAg（ ＋ ） serum show a higher rate than that in only HBeAg（ - ） serum（ P 〈 0.01）. trRNA have a high detection rate in HBV occult infection patient （44.0 % ）, and were found a high coincidence rate in serum and liver tissue of the same patient. Conclusion HBV transcript shows a close relationship with the end-stage liver disease. It was potentially possible that trRNA was a new marker of serum for the diagnosis of HBV infection.