β-纤维蛋白原五种基因多态性与脑梗死的关系

The relationship between the five β-fibrinogen gene polymorphisms and cerebral infarction

目的探讨血浆β纤维蛋白原水平及148C/T、249C/T、455G/A、448G/A、1689T/G基因多态性与脑梗死的相关性。方法运用PCR限制性内切酶片段长度多态性技术,检测脑梗死组132例、其他神经疾病对照组79例和正常老年对照组92例的血浆纤维蛋白原水平及5种基因多态性。结果脑梗死组的纤维蛋白原水平明显高于正常老年组和疾病对照组(P<0.05)。在脑梗死组、疾病对照组和正常老年组中,148C/T、455G/A基因多态性变异组的纤维蛋白原水平均高于无变异组(P<0.05)。而3组之间5种突变基因的基因型频率、等位基因频率差异均无统计学意义(P>0.05)。结论脑梗死作为多因素疾病,血浆纤维蛋白原水平升高是脑梗死的危险因素之一。T148、A455等位基因可引起血浆纤维蛋白原水平升高。

Objective To investigate the relationship among β-fibrinogen （Fg） concentration, related gene polymorphisms （ including -148C/T,-249C/T,-455G/A, 448G/A, 1689T/G ） and cerebral infarction. Methods Fg level and its five gene polymorphisms were analyzed with by polymerase chain reaction-restriction fragment length polymorphism in 132 patients with cerebral infarction, 79 patients with other neurological diseases and 92 healthy elders. Results The plasma Fg level in cerebral infarction patients was significantly higher than that in the patients with other neurological diseases or healthy elders （ P 〈0. 05）. In the three groups, the plasma Fg levels in individuals with T-148 and A-455 alleles were higher than those in individuals without T-148 and A-455 alleles （P 〈 0. 05 ）. However, there were no statistically significant differences in the genotype and allele frequencies in the five mutation gene polymorphisms among the three groups （ P 〉 0. 05 ）. Conclusions Cerebral infarction is a multifactorial disease and an increased Fg level is a risk factor for cerebral infarction. T-148 and A-455 allele can lead to elevated Fg concentration.