摘要
目的研究DNA转录与修复相关基因的表达与卵巢癌顺铂耐药性之间的关系。方法以卵巢癌亲本细胞系COC1为对照,应用cDNA微阵列检测其顺铂耐药细胞系COC1/DDP中DNA转录与修复相关基因的表达。结果和结论与COC1细胞相比,COC1/DDP细胞有143个基因的表达水平发生改变,其中共有20个DNA转录与修复相关基因的表达发生改变,表达上调的有13个基因,表达下调的有7个基因,包括CAD、DDB1、DDIT4等大量DNA修复基因以及一般转录因子RNA多聚酶Ⅰ、Ⅱ基因和DHX9、DDX3X基因的表达上调以及MLH1和BTF3基因的低表达,提示COC1/DDP细胞对顺铂的耐药性与DNA转录和损伤修复能力的异常有关。
Objective To study the molecular mechanism underlying cisplatin resistance in ovarian carcinoma by detecting the expressions of DNA transcription-and repair-related genes in cisplatin-resistant human ovarian carcinoma COC 1 cell line. Methods The differential expression of DNA transcription- and repair-related genes between the parental COC1 and cisplatinresistant COC1/DDP cell line was determined using cDNA microarray. Results and Conclusion Compared with COC 1 cells, 143 genes in COC1/DDP cells showed significant differential expression, among which 20 were DNA transcription- and repair-related genes including 13 significantly up-regulated genes and 7 down-regulated ones. Abnormality of DNA transcription and repair might be involved in the development of cisplatin resistance in COC 1/DDP cells.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第12期1478-1481,共4页
Journal of First Military Medical University
基金
国家自然科学基金(39870813)~~
