新型聚乳酸复合支架材料在组织工程骨构建中的实验研究

The Experimental Study of Modified Polylactic Acid Scaffoldings in Preparation of Tissue-Engineered Bone

目的:探索新型聚乳酸复合支架材料对兔骨髓基质细胞(bone marrow stromal cells,BMSC)生物学行为的影响及组织工程骨的构建方法。方法:实验组选择兔骨 MSC 诱导生成成骨细胞,接种于改良型聚乳酸复合新材料,包括新型聚乳酸(polylactic acid,PLA)、聚乳酸-乙醇酸共聚物(polylactide-co-glycolide,PLGA)、聚乳酸-乙二醇共聚物(polylactic acid-polyethylene glycol block copolymers,PLA-PEG)复合制作的三维支架中培养,用无机材料磷酸三钙(tricalcium phosphate,TCP)作为对照组,观察种子细胞在支架材料上的吸附迁移、生长增殖情况。结果:成骨细胞在新型三维支架材料表面分布较为均匀,生物学行为活跃。结论:可以通过 MSC 诱导获得成骨细胞。PLA、PLGA、PLA-PEG、TCP 具有良好的生物相容性,可作为支架材料与成骨细胞共同培养,获得具有成骨能力的三维立体结构组织工程骨。

Objective： To investigate the effect of modified PLA scaffoldings on biological behavior of bone marrow stromal cells and the procedure for fabricating tissue-engineered bone. Methods： MSC were induced into osteoblasts and cultured combined with polylactic acid （PLA） .PLGA .PLA-PEG in vitro, the morphological characters and cell proliferation were detected. Results： Induced osteoblasts attached to the modified scaffoldings, and normally grew, proliferated, and functioned actively. Conclusion： The scaffoldings materials, including PLA .PLGA ,PLA-PEG.TCP have good bioeompatibility, and could be cultured with osteoblasts in vitro to get tissue-engineering bone.