Ach对胃腺癌G_0蛋白α亚单位的影响

Effect of Ach on asubunit of G_0 protein in the grastric adenocarcinoma cells.

目的了解Ach对胃腺癌细胞G0蛋白α亚单位表达的影响,探讨其在肿瘤发生、发展中所起的作用。方法采用免疫组织化学SP方法,检测培养的胃腺癌细胞和正常胃粘膜上皮细胞中G0蛋白α亚单位的表达。结果与正常胃粘膜上皮细胞比较,胃腺癌细胞G0蛋白α亚单位呈强阳性表达。定量分析显示其数值显著增高,数密度增大。在Ach作用下,胃腺癌细胞和胃粘膜上皮细胞G0蛋白α亚单位均表现为数量增多,数密度与面密度增大(P<0.01),阿托品可阻断Ach对两种细胞的作用。结论Ach有增强细胞内信息传递通路的作用,G0蛋白参与了胃腺癌的发生、发展过程。

Objective To find out effect of a subunit expression of G0 protein in the gastric adenocarcinoma cells and to discuss their effect on the occurrence and progress of the tumor. Methods Expression of αsubunit in the gastric adenocarcinoma and normal mucosa eoithelial calls were detected by means of immunohistochemical staining method. Results Expression of G0 protein in the gastric adenocarcinoma was stronger positive compare with that of the normal cells. Its numbers were increased and number density was enlarged by quantitative analysis. Ach in the concentration, increased number and surface density of the αsubunit of G0 protein in the normal grastric and adenocarcinoma cells increased （P〈0.01）. Effect of the Aeh can be blocked by atropine. Conclusion Ach could enhance course of information transmission in the cells and Go protein could play certain role in the occurrence and progress of the gastric adenocarcinoma.