家蚕品种P50和C108后部丝腺的表达序列标签及单核苷多态性比较

Comparison of ESTs and SNPs From Posterior Silkgland of Silkworm Bombyx mori Between the Strain P50 and C108

利用BLAST软件对家蚕品种P50和C108的4眠及5龄幼虫后部丝腺的表达序列标签(EST)进行比较,从分子水平证明了5龄期后部丝腺蛋白合成比4眠期更加活跃。运用S im4和D iffseq工具就EST比对结果进一步分析,在两个家蚕品种中共发现有1 584个单核苷酸多态(SNP),其中颠换型变异的SNP达820次,占51.77%,转换型变异的SNP达764次,占48.23%;发现有2 776个插入/缺失(InDel)变异,其中1 bp的InDel占了48.56%,小于27 bp的InDel达到了90%以上。研究结果从基因组水平上阐明了这两个家蚕品种间的多样性。

The expressing sequence tags （ESTs） from the fourth molters and fifth instar silkworm larvae of strain P50 and C108 were analyzed with the BLAST sofeware. The results confirmed in the molecular level that the protein synthesis is more active in the fifth-instar than that in the fourth molting. Totally 1 584 candidate single nucleotide polymorphisms （SNPs） were identified with Sim4 and Diffseq tools from the ESTs, of which there were 820 times of transvertions （51.77%） and 764 times of transitions （48.23%）. And 2 776 insertions/deletions（InDels） were recognized, most of them were shorter than 27 bp （90%） with the 48.56% of 1 bp InDels. The above results have provided the data to illustrate the diversity of the two silkworm strains in the genomic level.