不同配比头孢噻肟与他唑巴坦体外抗菌作用

In vitro activities of different ratios from matching cefotaxime/ tazobactam

目的 研究不同配比的头孢噻肟／他唑巴坦外抗菌作用。方法 测定568 株临床分离菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。观察细菌接种 量、培养基pH和血清蛋白含量对体外抗菌活性的影响。结果 不同配比头孢 噻肟与他唑巴坦的抗菌活性无明显差异。与头孢噻肟单剂相比,对革兰阴性菌 的MIC90为0．25-16 mg·L-1,活性提高1-32倍,对葡萄球菌MIC50为0．12- 16 mg·L-1,活性提高1-32倍,MIC90为1-32 mg·L-1,活性提高2-8倍。结 论 头孢噻肟与他唑巴坦复方制剂体外抗菌作用远高于单剂头孢噻肟。3种 (1:1,3:1,5:1)配比抑酶抗菌作用相当。

Objective To study the in vitro antibacterial activities of cefotaxime and different formulations of cefotaxime/tazobactam. Methods Minimal inhibitory concentrations （MIC）and minimal bactericidal concentrations （MBC） were determined on 568 clinical isolates. Observe the influences of the inoculum size, pH and concentration of serum protein on antibacterial activities of cefotaxime/tazobactam. Results The antibacterial activities of cefotaxime/tazobactam between different ratios from matching havn＇ t significant difference. Compared to that of cefotaxime alone, MIC90 of cefotaxime/tazobactam against gram negative bacterium was 0. 25 - 16mg·L^-1, 1 -32 times higher than that of cefotaxime.. MIC90 of cefotaxime/ tazobactam against Staphylococcus sp. was 0. 125 - 16mg·L^-1 , 1 - 32 times higher than cefotaxime, MIC90 was 1 - 32 mg·L^-1,2 - 8 times higher than cefotaxime. Conclusion Antibacterial activities of different formula cefotaxime/tazobactam higher than cefotaxime significantly. The antibacterial activity of cefotaxime/sulbactam （5：1） is equal to that of 1：1 and 3：1 ratios.