多羟基双萘醛提取物WCT抗病毒的生理病理学研究

Antiviral physiopathology of WCT extracts of polyhydroxy dinaphthaldehyde

本文研究了多羟基双萘醛提取物WCT处理西葫芦后,对西瓜花叶病毒的防效和抗病毒相关酶活性及超微结构变 化。结果发现,先喷WCT 24 h后接种的相对预防效果为83．7％,高于先喷病毒A 24 h后接种的预防效果和先接种24 h后 喷WCT的治疗效果。WCT预防处理能在喷药后第3 d或第9 d相对于接种未喷药处理的PPO、POD和PAL的活性显著 提高,且3种酶活性随时问推移都有一个上升一下降-上升的交替起伏的变化趋势;能诱导西葫芦在Rf值为0．33、0．36、 0．43处多出3条新的PPO同工酶带,能增强2条在Rf值为0．73和0．79的POD同工酶带。WCT治疗处理酶活性变化规 律与接种未喷药处理的对照相近,对寄主植物没有诱导作用。在西葫芦发病初期,相对于接种未喷药处理的对照,WCT预 防处理的西葫芦叶肉细胞内病毒内含体数量减少,内含体的形状主要以风轮状为主,叶绿体和线粒体没有解体现象,且叶 绿体中淀粉粒膨大积累,叶绿体附近嗜锇颗粒增多。WCT治疗处理与接种未喷药处理的几乎没有明显差异。

After squash was sprayed with WCT, the relative biological control efficacy on Watermelon mosaic virus （WMV） ,antiviral enzyme activity and changes of ultrastructure of squash were studied in the greenhouse. The results indicated that protective efficacy of WCT on squash inoculated WMV after spraying WCT for 24 h was 83.7% , higher than that of Virucide A and therapeutic efficacy of squash spraying WCT after WMV inoculation for 24 h. The treatment of WCT protection could remarkably increase the activities of PPO, POD and PAL on the 3rd or 9th day compared with the untreated CK , and the activities of all three enzymes had a tendency of alternately fluctuanting change. It induced squash producing three new isoenzyme spectrums of PPO at Rf 0.33, 0.36,0.43 and enhanced isoenzyme lane of POD at Rf 0.73 and 0.79 compared with the untreated CK. The activity change of three enzymes of WCT therapy treatment resembled the untreated CK, which had no inducing function to host plants. At initial stages of virus symptoms , the number of virus inclusions reduced in the squash cells protectively treated by WCT , the shape of inclusions was mainly pinwheel, the chlorophyll and mitochondria was normal. The granuloses swelled and accumulated in chlorophyll, and the osmiumphile bodies increased near chlorophyll. The therapeutic treatment had no remarkable difference.