从青霉菌丝体中提取核糖核酸的研究

Extraction of RNA from waste Penicillium biomass

文中研究了从青霉菌丝体中提取纯化核糖核酸的工艺条件。采用盐碱法破碎细胞,调抽提液pH值至4.6以沉淀部分蛋白质,然后用中性蛋白酶AS1.398处理分级沉淀后的上清液,详细考察了蛋白酶用量、反应温度、初始pH值和反应时间对核酸纯度的影响。酶解液经截留相对分子质量为6.0×104的超滤膜过滤后,核糖核酸的纯度和得率分别是72.1%和0.42%。

The conditions of extraction and purification of RNA from waste Penicillium biomass were studied. A salt and alkali method was used for cell disruption, the pH value of extraction solution was adjusted to 4.6 to precipitate part of protein, and the supernatant after fractional precipitation was treated with neutral protease AS1. 398. The amount of AS1. 398 neutral protease, reaction temperature, initial pH value and the hydrolytic time were explored. Then the hydrolysate was treated with ultrafiltration membrane（MWCO is 6.0 × 10^4）, and the purity and the yield of RNA was 72.1% and 0.42 %, respectively.