灯盏乙素对过氧化氢致PC12细胞损伤的抗氧化作用

Antioxidant effect of scutellarin on PC12 cell injury induced by hydrogen peroxide in culture

目的研究灯盏乙素对过氧化氢(H2O2)诱导PC12细胞损伤的保护作用。方法建立H2O2致PC12细胞损伤模型,倒置相差显微镜下进行一般形态学观察,化学比色法测定乳酸脱氢酶(LDH)释放量及细胞培养液和细胞内丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果100μmol/L H2O2诱导PC12细胞4 h,细胞呈现明显损伤形态,LDH释放量增加,细胞培养液和细胞内MDA含量升高,SOD活性降低。灯盏乙素可明显改善形态学损伤,显著降低LDH释放量、细胞培养液及细胞内MDA含量,提高SOD活性。结论灯盏乙素对H2O2诱导PC12细胞损伤具有保护作用,其作用机制可能与提高PC12细胞的抗氧化能力有关。

Purpose To investigate the antioxidant effect of scutellarin on PC12 cell injury induced by hydrogen peroxide in culture. Methods The PC12 cells injury was induced by H2O2 in culture. Morphological changes of PC12 cells were visualized under light microscope. Lactate dehydrogenase （LDH）, malondialdelyde （MDA） and superoxide dismutase （SOD） in the culture medium and MDA, SOD in the cell were measured by spectroscopy respectively. Results After being exposed to 100μmol/L H2O2 for 4 hours, typical morphological characteristics of injury were observed. The level of LDH and MDA was increased significantly while SOD level was decreased significantly in H2O2 induced group compared with that in the sham group. Scutellarin can decrease LDH, MDA content and increase SOD activity significantly. Conclusion Scutellarin has protective effect on PC12 cell injury induced by hydrogen peroxide in culture. The protective effect may be related to protecting cell membrane, to increasing the activity of clearance enzyme of free radical and to inhibiting lipid peroxidation.