青蒿琥酯对佐剂性关节炎大鼠踝关节滑膜的NF-κB、bcl-2表达的影响

The effect on the expression of NF-κB and bcl-2 in synovium of joint of sodium artesunate in treating adjuvant arthritis of rats

目的:观察青蒿琥酯对佐剂性关节炎大鼠踝关节滑膜的病理形态和滑膜细胞的NF-κB、bcl-2表达的影响。方法:42只♂性Wistar大鼠随机分为5组,空白组(8只)、模型对照组(8只)、青蒿琥酯Ⅰ组(8只,40mg.kg-1)、青蒿琥酯Ⅱ组(8只,20mg.kg-1)、青蒿琥酯加甲氨蝶呤组(10只,青蒿琥酯20mg.kg-1、甲氨蝶呤每3d0.5mg.kg-1)。采用大鼠右后足跖皮内注射完全性福氏佐剂0.1mL,致炎形成佐剂性关节炎(AA)模型,于造模12d后开始给药。给药12d后处死动物,组织切片HE染色观察大鼠踝关节滑膜的组织形态学变化,免疫组化ABC法检测滑膜组织中核转录因子NF-κB、凋亡相关蛋白bcl-2的蛋白表达水平。结果:青蒿琥酯能明显减轻滑膜细胞的增生,减少滑膜层和滑膜下层淋巴细胞浸润。青蒿琥酯Ⅰ组、青蒿琥酯Ⅱ组及青蒿琥酯加甲氨蝶呤组关节滑膜细胞的bcl-2和NF-κB表达明显低于模型组,差异有显著性(P<0.05)。结论:青蒿琥酯可显著改善佐剂性关节炎大鼠的滑膜病变,降低滑膜细胞的bcl-2和NF-κB的蛋白表达。

OBJECTIVE To observe the effect on the expression of NF-κB and bcl-2 in synovium of joint of sodium artesunate in treating adjuvant arthritis of rats. METHODS 34 rats were modelled to adjuvant arthritis with Freund＇s complete adjuvant apart from normal group 8, animals were randomly divided into the normal group 8, the model group （the control group 8）, the sodium artesunate Ⅰ group 8, the sodium artesunate Ⅱ group 8, and the sodium artesunate plus methotrexate （MTX） group 10. The immunity and histochemistry character of the joint under microscopy and the expression level of NF-κB and bcl-2 in synovium of joint were recorded after treating with sodium artesunate or sodium artesunate plus MTX. RESULTS Compared with the control group, the expression level of NF-κB and bcl-2 in synovium of joint was lower significantly （P〈0. 05） in the sodium artesunate Ⅰ group and the sodium artesunate plus methotrexate （MTX） group. CONCLUSION Sodium artesunate can decrease the expression level of NF-κB and bcl-2 in synovium of joint of adjuvant arthritis rats, which indicates that sodium artesunate can be used to treat adjuvant arthritis of rats. The subject provides determinate experiment gist for treating RA with artemisinir.