高效液相色谱法测定半夏中琥珀酸含量

HPLC determination of succinic acid in Rhizoma Penilliae

目的:建立半夏、掌叶半夏和水半夏等中药品种中琥珀酸含量的HPLC测定法.方法:用Inertsil C18色谱柱(4.6 mm×250 mm,4μm),以磷酸盐缓冲液(pH 2.5)为流动相,检测波长为210 nm,柱温为40℃,流速为1.0 mL·min-1.结果:琥珀酸在样品中的平均回收率为98.67%,RSD为1.8%.半夏样品中的琥珀酸含量,以干燥品计为0.0038%～0.0304%,掌叶半夏中为0.0110%～0.0559%.结论:该方法可供半夏等中药中进行琥珀酸的含量测定时参考.

OBJECTIVE To establish an HPLC method to determine the content of succinic acid in Rhizoma Penilliae, Rhizoma Penilliae Pedatisectae ,Rhizoma Typhonii Flagelliforme and other correlative herbs. METHODS Using column Inertsil C18（4μm, 4. 6 mm× 250 mm）and phosphate buffer solution（pH 2. 5） as mobile phase, with the detection wavelength of 210 nm, column temperature of 40℃ and rate of flow 1.0 ml·min^-1. RESULTS The average recovery rate of succinic acid in samples was 98. 67% with RSD of 1.8 %. The content of succinic acid in Rhizoma Penilliae and Rhizoma Penilliae Pedatisectae was 0. 0038%～0. 0304% and 0. 0110～0. 0559% respectively. CONCLUSION This method can be used for determination of succinic acid in Rhizoma Penilliae and other correlated herbs.