改进的柱前衍生高效液相色谱法测定人血中丙戊酸钠

Determination of sodium valproate in serum by HPLC after precolumn derivatization

目的:建立高效液相色谱法测定人血清中丙戊酸钠浓度的方法。方法:血样用正戊烷提取,以环己烷羧酸为内标,α溴苯乙酮为衍生化试剂,用高效液相色谱法测定丙戊酸钠血药浓度。采用KromasilC18柱(150mm×4.6mm,5μm),流动相为甲醇水(72∶28),检测波长为266nm,流速1.2mL·min-1。结果:血清中丙戊酸钠线性范围为15～200mg·L-1,平均回收率为101.0%,日内、日间RSD<5%。结论:本法快速、简便、准确,适用于临床常规监测需要。

OBJECTIVE To determine sodium valproate in serum by high performance liquid chromatography（HPLC）. METHODS Serum sample（100μL） was extracted with 2 mL n-pentane, cyclohexanecarboxylic acid was selected as an internal standard, α- bromoacetophenone as derivative reagent. An UV detector （266 nm） and Kromasil C18 column （150 mm×4. 6 mm, 5μm）was used. The mobile phase consisted of methanol-water（72：28）, The flow rate was 1.2 mL·min^-1. RESULTS The linear range of sodium valproate was 15-200 mg·L^-1, The mean recovery was 101.0 %, The relative standard deviation（RSD）of inter-day and intra-day for sodium valproate was less than 5 %. CONCLUSION The method is accurate, sensitive and suitable for clinical routine monitoring.