高效液相色谱-荧光法测定人血浆替米沙坦浓度

Determination of telmisartan concentration in human plasma by HPLC-FLU method

目的:建立血浆中替米沙坦浓度的高效液相色谱分析方法。方法:采用高效液相色谱荧光法,对流动相的组成进行了考察,确定乙腈水醋酸三乙胺(60∶40∶0.4∶0.3,pH4.2)为流动相洗脱;流速:1mL·min-1,激发波长:295nm,发射波长:385nm。内标法定量,血浆样品经简单的预处理后进样,血浆中内源性物质不干扰替米沙坦的测定。结果:替米沙坦在2.5～120.0μg·L-1范围内线性关系良好(r=0.9994),最低检测限为1.25μg·L-1;低、中、高3种浓度样品的平均方法回收率分别为98.02%,102.13%,100.57%;精密度分别为4.06%,6.17%,2.38%。结论:本方法准确、快速、简便、灵敏度高,适合替米沙坦的药动学的研究。

OBJECTIVE To establish an analytical method for determination of telmisartan in human plasma. METHODS HPLCFLU method was applied to measure telmisartan concentration. The mobile phase composition was selected to get a good separation and peak shape. Finally, the elute solution was prepared as follow： acetonitrile-water- acetic acid-triethylamine（60：40：0. 4：0. 3; pH 4. 2） ; the flow rate was 1 mL·min^-1. The excitation and emission wavelength was 295 nm and 385 nm, respectively. Internal standard method quantified telmisartan concentration in plasma. Prior to analysis, the plasma sample was pretreated easily through onestep extraction. No intrinsic substances interfered analysis of telmisartan. RESULTS A good linear relationship was observed from 2. 5-120. 0μg·L^-1 , the detection limit was 1.25μg·L^-1. The relative recovery rate of three concentration samples were 98. 02%, 102. 13%,100. 57%; and the precise were 4. 06%,6. 17%,2.38%,respectively. CONCLUSION The method is accurate, simple, rapid and sensitive; it can be used for the pharmacokinetics research of telmisartan.