大鼠重症急性胰腺炎及白藜芦醇治疗时血清IL-6及TNF-α测定的意义

Significance of Determination of Changes of Serum IL-6 and TNF-α Levels in Rat Models of Severe Acute Pancreatitis(SAP) Treated with Reseratrol

目的:探讨重症急性胰腺炎(SAP)时大鼠IL-6、TNF-α的变化及白藜芦醇对其影响。方法:将实验大鼠分为假手术组、SAP组、白藜芦醇治疗1组(5mg/kg)、治疗2组(10mg/kg)、治疗3组(20mg/kg),制作SAP的动物模型后,3h、6h、12h剖杀大鼠,记录腹水量和性质,应用放射免疫分析测定血清IL-6、TNF-α浓度,取胰腺组织,观察病理变化。结果:①术后12h假手术组、治疗2组、3组生存率为100%,与SAP组(41.7%)生存率比较有显著差异(P<0.05),治疗1组生存率为50%;②治疗组2、3各时段腹水量与SAP组和治疗1组比较明显减少(P<0.05);③治疗组2、3各时段IL-6水平与SAP组和治疗1组比较明显降低(P<0.01),治疗组1与SAP组比较IL-6水平下降不明显;④治疗组2、3与SAP组各时段比较,TNF-α水平明显降低(P<0.05);⑤治疗组2、3术后6h、12h胰腺细胞坏死,评分较SAP组降低(P<0.05)。结论:①SAP时血清IL-6、TNF-α水平均显著增高,其在SAP进展中有重要作用;②白藜芦醇可明显降低,SAP大鼠IL-6、TNF-α水平,抑制炎症介质的释放和作用。

Objective To investigate the changes of serum IL - 6 and TNF - α levels in rat models of SAP and the treatment effect of reseratrol. Methods Five groups （n = 24 each） of rat models of SAP were prepared with intrapancreatic injection of sodium tauroglycholate. One group were left untreated, the other three groups were treated with reseratrol at low （5mg/kg）, medium （10mg/kg） and high （20mg/kg） dose respectively. Animals from each group were sacrificed at 3h, 6h, and 12h after establishment of the models, serum IL -6, TNF -α levels, amount and character of peritoneal exduates as well as gross and microscopic pathology of pancreas were examined. A sham operation group served as controls. Results ① Survival rate at 12h in the sham operation group as well as the groups treated with medium or high doses of reseratrol was 100%, which was significantly higher than that in the untreated SAP group （41. 7% ）. Survival rate in the group treated with low dose of reseratrol was 50%. ② Amounts of peritoneal exudate in the groups treated with medium and high dose of reseratrol were significantly less than those in the untreated and low dose groups at all times （ P 〈 0. 05 ）. ③ The serum IL -6 levels in the medium and high dose groups were significantly lower than those in the untreated and low dose groups （P 〈 0, 01 ）. Decrease of serum IL - 6 levels in the low dose group was not significant （ vs untreated group）. ④ TNF -α levels in the medium and high dose groups were significantly lower than those in the untreated group （P 〈 0. 05 ）. ⑤ Scores of pancreatic necrosis in the medium and high dose groups were significantly less than those in the untreated group （P 〈 0. 05）. Conclusion ① Serum IL -6 and TNF - α levels in creased markedly in the rat models of SAP. ② Reseratrol treatment could markedly decrease the serum IL - 6 and TNF - α levels, with inhibition of the imflarnmatory process.