槟榔碱对小鼠酒精急性中枢抑制作用的影响

EFFECTS OF ARECOLINE ON CENTRAL SUPPRESSION IN MICE TREATED ACUTELY WITH ETHANOL

目的:探讨非选择性毒蕈碱受体(M受体)激动剂—槟榔碱,对小鼠酒精急性中枢抑制作用的影响。方法:测定小鼠的自主活动,观察槟榔碱对酒精诱导的小鼠低活动性的影响。建立酒精诱导小鼠翻正反射消失(loss of therighting reflex,LORR)的模型,观察槟榔碱对LORR潜伏期和持续时间的影响。结果:酒精(1.0、2.0、3.0 g.kg-1)和槟榔碱(0.25、0.51、.0 mg.kg-1)均可剂量依赖性地抑制小鼠的自主活动,但槟榔碱对酒精诱导的小鼠低活动性无影响。槟榔碱(0.25、0.5、1.0 mg.kg-1)对酒精诱导小鼠LORR的潜伏期无影响,但可显著缩短LORR的持续时间。结论:槟榔碱可以拮抗酒精诱导小鼠LORR的药理作用,提示槟榔碱可能具有一定的醒酒作用。

Objective： To investigate the effects of arecoline （AREC）, non- selective muscarine receptor agonist, on acute ethanol（EtOH）- induced central suppression in mice. Mete： Locomotor activity was detected to observe the effect of AREC on EtOH-induced hypoactivity after co-administration of AREC with EtOH in mice. Mice were induced loss of the righting reflex （LORR） to EtOH （4.0 mg·kg^-1）, and administered with the combination of AREC and EtOH to observe the effects of AREC on the latency to and duration of EtOH-induced LORR. Results： A single intraperitoneal administration of EtOH （1.0,2.0, 3.0mg·kg^-1） or AREC （0.25,0.5,1.0 mg·kg^-1） dose-dependently inhibited the locomotor activity in mice, but there was no effect of AREC on EtOH- induced hypoactivity. AREC （0.25,0.5,1.0 mg·kg^-1） significantly shortened the duration of EtOH-induced LORR, although it had no effect on the latency to LORR. Conclusion： AREC can antagonize EtOH-induced LORR in mice. Thus, AREC may alleviate ethanol drinking.