线粒体F型ATP合成酶的制备方法研究与探讨(英文)

Methods in the Preparation of F-Type ATP Synthase

科研工作者们在过去的50年前赴后继的工作中深入研究了ATP合成酶的功能,并努力尝试解析该酶的空间结构以便能够从结构基础上对ATP合成酶的催化机理进行阐明;但蛋白的纯化工作却一直是困扰研究顺利进展的最大障碍.蛋白的纯化技术是与特定阶段科技发展及科研工作者思维模式的直接反应,因而是一门不断发展的艺术.文章主要介绍了ATP合成酶的提取与纯化工作,在详细对比了现有方法的基础上,给出了纯化此酶复合体的详细方案和线粒体、亚线粒体、ATP合成酶的提取与纯化方法;并在方法讨论的基础上对线粒体F型ATP合成酶的研究提出了前瞻性的方案.

As the energy currency of the cell,ATP is mainly produced by the catalysis of ATP synthase in the mitochondria. In the past 50 years, scientists have done much on ATP synthase＇s structure and its function already, but people have known more about function but fewer about the mechanism,so there is much more work to do.High purified ATPase which should come first is the key factor in the study of enzyme＇s structure and catalytic mechanism. So isolation of the ATP synthase has become the primary work for the researchers who want to study it. This article has summarized and introduced some valuable methods in the isolation of mitochondria and submitochondria, and purification of ATP synthase, and the assay of the enzyme＇s activities in details respectively.