摘要
目的:观察登革2型病毒衣壳蛋白(D2C)与葡萄球菌核酸酶(SN)融合蛋白在哺乳动物细胞中对登革2型病毒增殖的影响.方法:通过基因重组构建可在哺乳动物细胞中表达融合蛋白D2C-SN的重组质粒pc/D2C-SN,同步构建pc/D2C-SN*用作对照.病毒感染BHK21细胞后,通过阳离子转染试剂将重组质粒导入感染细胞,在此基础上评价融合蛋白D2C-SN抗登革病毒感染的治疗效果.结果: 融合蛋白D2C-SN能够在哺乳动物细胞中表达,对宿主细胞没有明显的毒性;与正常BHK21细胞相比较,可导致病毒感染性滴度降到原来的1/60～1/12.结论:融合蛋白D2C-SN在细胞水平能够有效抑制登革病毒的增殖,有可能成为潜在的抗登革病毒感染的治疗性药物.
Objective：To observe the therapeutic effect of D2C-SN fusion proteins against dengue 2 virus infection in vivo. Methods：The eukaryotic vector expressing pc/D2C-SN was constructed by genetic recombination, and pc/D2C-SN^＊ was simultaneously constructed as control. The recombinant plasmids were then transfected into BHK-21 cells pre-infected with wild dengue 2 virus, and the effect on dengue virus multiplication was evaluated by examining the production of infectious virions, Results： The intracellular expressed fusion protein D2C-SN had no cytotoxicity on BHK cells and resulted in a reduction by about 12- to 60- fold in infectious titers compared with wild dengue 2 virus infection. Conclusion：The fusion proteins D2C-SN could obviously inhibit the multiplication of dengue 2 virus in mammalian cells, and may become a poten- tial drug against infection of dengue virus.
出处
《军事医学科学院院刊》
CSCD
北大核心
2005年第6期506-508,511,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家自然科学基金资助项目(3010006)
