摘要
采用SDS-PAGE方法,通过对5个亲本间杂交获得的F2群体每一单株的F3籽粒样本及其亲本进行小麦高分子量谷蛋白亚基HMW-GS组成分析,并对每一F2单株上F3籽粒群体的高分子量谷蛋白亚基组成与其籽粒蛋白质含量、SDS-沉降值的关系进行研究,分析比较黄淮麦区出现频率较高的7个亚基或亚基对的品质效应.结果表明:小麦高分子量谷蛋白亚基组成不同群体间籽粒的蛋白质含量和SDS-沉降值基本达到显著或极显著水平.优质亚基表现为:1、7+8、14+15和5+10亚基.因此,黄淮麦区小麦育种应加强对这些优质亚基的引入和利用,特别是对14+15和5+10亚基的引入和利用.
The HMW-GS compositions of the sampled Fa kernels and parental kernels of each plant in the F2 populations formed by crossing five parents were assayed by SDS-PAGE. The relationships of the HMW- GS compositions of the F3 kernels of each F2 plants with the protein contents and SDS-sedimentation value of these kernels were studied and seven bigh-frequency subunits and their quality effects in Huanghuai Area of Wheat Production were comparatively probed. The results showed that different populations of wheat with different HMW-GS compositions by and large appeared to significantly or extremely significantly differ in the protein contents and SDS-sedimentation values of their kernels. The quality subunits or subunit combinations were subunit 1 ,subunit 7+subunit 8,subunit 14+subunit 15 and subunit 5+subunit 10. The therefore,more attention should be paid to the introduction and exploitation of these subunit or subunit combinations in wheat breeding for Huanghuai Area of Wheat Production.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第12期2443-2446,共4页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省科技攻关项目2004
OK1-G3
杨凌生物技术育种中心专项基金资助项目2002A-4
关键词
小麦
高分子量谷蛋白亚基
品质效应
wheat
high molecular weight glutenin subunit
quality effect