小麦高分子量谷蛋白亚基效应的比较研究

Comparison of the Effects of High Molecular Weight Glutenin Subunits in Wheat

采用SDS-PAGE方法,通过对5个亲本间杂交获得的F2群体每一单株的F3籽粒样本及其亲本进行小麦高分子量谷蛋白亚基HMW-GS组成分析,并对每一F2单株上F3籽粒群体的高分子量谷蛋白亚基组成与其籽粒蛋白质含量、SDS-沉降值的关系进行研究,分析比较黄淮麦区出现频率较高的7个亚基或亚基对的品质效应.结果表明:小麦高分子量谷蛋白亚基组成不同群体间籽粒的蛋白质含量和SDS-沉降值基本达到显著或极显著水平.优质亚基表现为:1、7+8、14+15和5+10亚基.因此,黄淮麦区小麦育种应加强对这些优质亚基的引入和利用,特别是对14+15和5+10亚基的引入和利用.

The HMW-GS compositions of the sampled Fa kernels and parental kernels of each plant in the F2 populations formed by crossing five parents were assayed by SDS-PAGE. The relationships of the HMW- GS compositions of the F3 kernels of each F2 plants with the protein contents and SDS-sedimentation value of these kernels were studied and seven bigh-frequency subunits and their quality effects in Huanghuai Area of Wheat Production were comparatively probed. The results showed that different populations of wheat with different HMW-GS compositions by and large appeared to significantly or extremely significantly differ in the protein contents and SDS-sedimentation values of their kernels. The quality subunits or subunit combinations were subunit 1 ,subunit 7＋subunit 8,subunit 14＋subunit 15 and subunit 5＋subunit 10. The therefore,more attention should be paid to the introduction and exploitation of these subunit or subunit combinations in wheat breeding for Huanghuai Area of Wheat Production.