急性脑静脉闭塞动物模型的改进

Improved model of acute cerebral venous occlusion:experimental study

目的对急性脑静脉闭塞动物模型加以改进,拟建立一个稳定的类似临床疾病的动物模型。方法家猫28只,随机分为3组(栓塞组18只,结扎组6只,假手术组4只),栓塞组和结扎组分别采用开颅上矢状窦穿刺注射醋酸纤维素聚合物(CAP)和上矢状窦(SSS)后份结扎制备急性脑静脉闭塞模型,术后1、3、6、12、24、48h对各组模型行MRI、CT检查,观察脑实质损害的动态变化,并和病理作对照研究。结果栓塞组16只猫造模成功。MR发现13只猫,CT发现12只猫脑实质内异常灌注区。大体病理学16只猫上矢状窦、桥静脉及皮层静脉内见CAP凝固呈铸型改变。显微病理学见病灶以血管源性水肿为主,12h后出现静脉性脑梗死、脑出血。结扎组和假手术组脑实质未见异常改变。结论上矢状窦穿刺注射CAP制备急性脑静脉闭塞模型方法可行,该模型适合于病理生理及影像学研究。

Objective To improve the model of acute cerebral venous occlusion and establish a stable animal model to be similar to clinical disease. Methods Twenty-eight cats were randomly divided into 3 groups, including embolism group （n=18）, ligationgroup （n=6） and sham operation group （n=4）. Embolism group and ligation group were performed respectively by injection of the cellulose acetate polymer （CAP） solution into the superior sagittal sinus （SSS） and by ligation of the posterior part of the SSS. MRI and CT were performed at an interval of 1, 3, 6, 12, 24 and 48 h after operation. Brain parenchymal lesions were observed dynamically and compared with pathological changes. Results Sixteen cats in embolism group were operated successfully. Abnormal perfusion areas of brain parenehyma were found in 13 cats by MRI and 12 cats by CT. There were cast-like materials formed by CAP within SSS, bridging veins and cortical veins in 16 cats. The microscopic changes were mainly vasogenie edema and followed by venous hemorrhagic infarction in 12 h. No abnormal findings were observed in ligation group and sham operation group. Conclusion Modeling of acute cerebral venous occlusion by injection of CAP solution into SSS is feasible. The model is suitable for pathophysiologieal and radiologieal studies of acute cerebral venous occlusion.