胶束电动毛细管色谱法测定减肥保健品中的西布曲明、吲达帕胺、丁脲胺和氯噻嗪

Determination of Sibutramine,Indapamide,Bumetanide and Chlorothiazide in Mass-reducing Tonic by Micellar Electrokinetic Capillary Chromatography

目的:建立了胶束电动毛细管色谱法同时测定减肥保健品中的西布曲明、吲达帕胺、丁脲胺和氯噻嗪的方法。方法:以10.0 mmol·L^(-1)磷酸盐缓冲溶液-15.0 mmol·L^(-1)SDS(含35%乙腈,V/V,pH 7.5)为电泳介质,未涂层弹性石英毛细管(50μm×48cm,有效长度为40 cm)为分离通道,检测波长为210 nm,压力进样(5kPa×10s)。结果:西布曲明、吲达帕胺、丁脲胺和氯噻嗪的线性范围分别为4.0～100.0 m·L^(-1),2.0～80.0 mg·L^(-1),2.0～80.0 mg·L^(-1),2.0～80.0 mg·L^(-1),检出限分别为1.0,0.5,0.5,0.5 mg·L^(-1),5次重复测定的相对偏差为2.8%～4.5%;样品加标回收率为89%～103%。结论:该法简便快捷,准确可靠,用于中药成分减肥保健品分析,结果令人满意。

Objective：To develop a method for determination of sibutramine,indapamide, bumetanide and chlorothiazide in weigh - reducing tonic by micellar electrokinetic capillary chromatography. Method： The running buffer was composed 10. 0 mmol · L^-1 phosphate and 15.0 mmol L^-1 SDS （ pH was adjusted to 7.5 with phosphoric acid）. The separation was performed on a fused -silica capillary （50 m 48 cm,40 cm of effective length）. The detection was set at 210 nm and 5 kPa 10 s pressure injection was used. Results：the linear ranges were 4. 0 - 1130. 0 mg · L^-1,2.0-80.0 mg · L^-1,2.0 -80.0 mg · L^-1,2.0 -80.0 mg · L^-1,with a detectim limits of 1.0,0.5, 0. 5,0. 5 mg · L^- 1 for sibutramine, indapamide, bumetanide and chlorothiazide, respectively. The relative standard devivatims were 2.8% -4.5% （n = 5 ）,and the average recoveries ranged from 89% to 103%. Conclusion：The method is simple, rapid, accurate and suitable for simultaneous determination of sibutramine, indapamide, bumetanide and chlorothiazide in weigh -reducing tonic with satisfactory results.