乳酸杆菌半连续式高密度培养的研究

High cell density culture of Lactobacillus sp by semi-continu ous way

研究了乳酸杆菌的半连续高密度培养,探讨更换新鲜培养基对菌体浓度、解除代谢产物抑制等的影响,结果表明,经3次培养后菌体浓度达到9.3×1012cfu/mL。并对3次培养过程进行动力学分析,设计了培养-分离耦合生物反应器,用于恒浊连续高密度培养。

This paper studies high cell density culture of Lactobacillus sp. by semi-continuous way, and discusses the effects of fresh cultural media on the number of Lactobacillus sp and relieving the inhibition by metabolic products. The results showed that the number of Lactobacillus sp was up to 9.3×10^12cfu/mL after three times fermentation. Then the culture -separation bioreactor was designed and its parameters calculated to realize high cell density culture with high productivity for large-scale production based on the dynamic analysis of semi -continuous course.