保加利亚乳杆菌分批发酵工艺研究

Study on the batch-fermentation of L.bulgaricus

研究得出保加利亚乳杆菌适宜的碳氮源分别为乳糖和牛肉膏,适宜的碳氮比例为6∶1。在10L发酵罐水平上探明了新型培养基的增殖效果,证实保加利亚乳杆菌在优化发酵培养基中42℃培养8h后的活菌数达到3.6×109cfu/mL,增殖效果是经10%脱脂乳培养后的1.5倍。同时发现,当发酵液中乳酸盐浓度高于37.2g/L会抑制菌体生长,亟待发展新技术来解决代谢产物遏制的难题。

In this study, it＇s found that the optimum carbon sources and nitrogen sources for L.bulgaricus were lactose and beef extract respectively, the suitable ratio of carbon/nitrogen sources was C：N=6：1. The promoting effect of the optimum medium was investigated in 10L jar-fermentor, it proved that L.b obtained its maximum yield at 8 hours and viable counts reached 3.6 ×10^9cfu/mL in the medium, which was as much as 1.5 times in 10% skim milk medium. At the same time, when the concentration of salt reached 37.2g/L, it would inhibit the growth of cells, so there is a need to develop new technology to solve such problem as soon as possible.