流动注射化学发光法测定诺氟沙星

Determination of Norfloxacin by Flow-Injection Chemiluminescence

在酸性条件下,Ce(Ⅳ)与诺氟沙星(NFLX)能产生弱的化学发光反应,十二烷基硫酸钠(SDS)对其 有较强的增敏作用。据此建立了一种简单、快速、町连续测定诺氟沙星的流动注射化学发光新方法。该法线 性范围7．78×10-8-5-82×106g·mL-1,检测限(3σ)为1．57×10-8g·mL-1,对1．94×106g·mL-1的 NFLX标准溶液平行测定10次,相对标准偏差为1．7％。该法成功应用于诺氟沙星胶囊的测定,还对其机理 进行了初步探讨。

Sodium dodecylsulfate（SDS） can greatly enhance the emission intensity of the weak chemiluminescence reaction of Ce（ Ⅳ ） with norfloxacin（NFLX） in acid medium. Based on this phenomenon, a simple and rapid new method for the continuous determination of norfloxacin was developed. The calibration graph is linear for 7.78 ×10^-8-5.82 ×10^-6 g·mL^-1, the detection limit（3σ） is 1.57 ×10^-8 g·mL^- 1, and the relative standard deviation （ n = 10, c = 1.94 ×10^-6 g·mL^-1 ） is 1.7 %. The method was successfully applied to the determination of norfloxacin in capsules. Additionally, the system＇s mechanism was discussed.