结节性甲状腺肿的傅里叶变换红外光谱体表测定

Nodular Goiter Surface Detection by FTIR Spectroscopy

利用ATR探头和中红外光导纤维红外光谱法在体表测定34个正常甲状腺和20个结节性甲状腺肿 的傅里叶变换红外光谱。并比较了二者之间13条谱带的27个指标的差异。结果表明:结节性甲状腺肿的体 表红外光谱中:(1)-2 925 cm-1谱带(-CH2的反对称伸缩振动)的峰位,-1 250 cm-1(P=O的伸缩振 动)谱带的峰位均明显地向低波数方向移动(P<0．05);(2)谱带的相对强度(谱峰高度)比值H1 740／H1 460, H1 160／H1 460和H1 160／H1 120较正常甲状腺明显降低(P<0．05);(3)H1 080／H1 460比值明显升高(P=0．008)。 这些差异是利用体表红外光谱诊断结节性甲状腺肿的基础。

A novel non-invasive diagnosis method of nodular goiter is proposed in the present study by recording FTIR spectra on the skin overlying thyroids using fiber optical technique and attenuated total reflection probe. FTIR spectra from 20 nodular goiters and 34 normal controls were collected. Twenty seven spectral variables of 13 bands including peak position and relative intensities were extracted from the FTIR spectra so that statistic work could be conducted using SPSS. The results demonstrate that peak positions of 2 925 and 1 250 cm^-1 both shifted toward lower wave number（P〈0.05） in the FTIR spectra of nodular goiter. The relative intensity ratios of H1740/H1460, H1160/H1460, and H1160/H1120 decreased significantly in FTIR spectra of nodular goiter （P 〈 0.05）. Inversely, H1080/H1460 increased significantly （P〈 0.05） in nodular goiter. The above statistic differences suggest that nodular goiter may produce some characteristic chemical substance which can diffuse onto the surface of skin and therefore be detectable using FTIR spectroscopy with fiber optic techniques. These differences are the basis of diagnosing nodular goiter by FTIR surface detection.