控制细胞内游离［Ca^（2＋）］的螯合剂BAPTA衍生物的合成及结构与性能关系

Synthesis of chelators BAPTA derivatives for controlling intracellular free calcium and relationship of the structure and properties

控制细胞内游离［Ｃａ２＋］用的七种螯合剂ＢＡＰＴＡ衍生物已合成成功。其中对［Ｃａ２＋］低亲和力的５，５＇－二硝基ＢＡＰＴＡ及中等亲和力的５，５＇二溴ＢＡＰＴＡ、５—溴—５＇—甲基ＢＡＰＴＡ已分别应用于控制不同体系的［Ｃａ２＋］测定．本文详述它们的合成方法、性能及苯环上不同取代基对ＥＸ、Ｅｍ、Ｋｄ值影响的规律性。

Seven chelators BAPTA derivatives for controlling cytosolic free [Ca2+] have been synthesized. Among these, the 5,5'-dinitro BAPTA with low affinity for [Ca2+] and 5,5'-dibromo BAPTA. 5-bromo-5'-methyl BAPTA with intermediate affinity for [Ca2+] have been used to control the determination of different systems [Ca2+]. The synthesis, properties and effects of the substituents on benzene ring to Ex、Em、Kd are seported.