小鼠宫腔内用醋酸棉酚的抗生育作用及其与钙调素的关系

Antifertility Effect of Intrauterine Injection of Gossypol with Its Relation to Calmodulin in Mice

于小鼠妊娠第３天每侧子宫角内注入０．１８ｍｇ醋棉酚混悬液（０．０５ｍＬ），对照组注入等量溶剂．结果表明，醋酸棉酚对小鼠有显著的抗生育作用，抗生育率为９３％，抗着床率为７７％．钙调素（ＣａＭ）含量的测定表明，实验组子宫组织的ＣａＭ含量明显低于对照组，证明子宫内ＣａＭ的降低是局部用棉酚抗生育作用的机理之一．

This experinent was carried out in mice three days after pregnaney. 0. 05ml. of 0. 18mg gossypol acetic acid suspension was injected into each uterine horn of pregnant animals and the same dosage was gived to animals of the control group.It was found that a single injection of small amount of gossypol acetie acid gave an antifertility rate of 93 % and an anti-implantation rate of 77% of the experimental group.By means of ELISA(Method),concentration of calmodulin was determined in uterus of experimental animals as well as those of the control group. The result showed that the concentration of CaM in uterus of the former ones wus markdely reduced in comparing with that of the latter group. It indicated that reduction of CaM content in uterus minght be one of the mechanism of antifertilily action of gossypol.