摘要
壳聚糖及其衍生物能很容易被脂肪酶解聚。当壳聚糖乳酸盐溶液与脂肪酶在２５℃下作用１０ｍｉｎ后，其溶液粘度被降低到初始值的３５％，分子量从约７００ｋＤａ减少到１３ｋＤａ（还原糖滴定法测定）。脂肪酶浓度在４．５×１０－３～９×１０－１ｇ／Ｌ范围内，解聚初速度与酶浓度成对数线性关系。Ｎ－羧甲基壳聚糖比壳聚糖更容易被脂肪酶解聚。
Chitosan and derivatives were depolymerized by lipase,with the molecular weight lowering down from ca.700kDa to 13kDa,as shown by the viscosity decrease of its lactate solutions and by the titration of the reducing sugars.Typically,the viscosity was lowered to 35/00 of the initial value after 10 min.Incubation with lipase at 25℃.Measurements taken in the lipase concentration range 4.5×10-3～9.0×10-1 g/L showed a logarithmic dependence of the initial velocity on the enzyme concentration.N-Carboxymethyl chitosan was an even better substrate.
