摘要
将盐生杜氏藻(6-4)光裂合酶基因Ds64PHR的编码序列构建到表达载体pET32a中,利用高效转化法将构建好的表达载体转入E.coli.CPD光裂合酶缺陷菌株SY2中,诱导表达(6-4)光裂合酶融合蛋白,对其功能进行验证.在含有Amp的LB平板上涂布相同数量的大肠杆菌,改变紫外照射强度、光照修复时间,统计最终的菌株存活率.经研究发现,在基因水平上,SY2中表达的盐生杜氏藻(6-4)光裂合酶具有修复紫外诱导损伤的功能,光照是修复功能实现的必需条件.
Expression plasmid, pET32a-Ds64PHR, which was constructed by inserting （6-4） photoproducts photolyase gene from Dunaliella salina into E. coll. expression vector pET32a, was transformed into CPDdeleted E. coll. SY2. Then the gene was induced to express proteins and the function of the photolyase gene Ds64PHR was identified. The same amount of E. coli was pushed on the LB-Amp plates and the UV dose or the visible-light-irradiated time was chosen as a variable. Analysis of the number of bacteria suggest that the （6-4） PD photolyase expressed in SY2 had a function of repairing UV-induced lesions at gene level and light was necessary to this course.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第6期1274-1278,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金项目(30270711)
