IL-2协同IL-12诱导人单个核细胞及其体外抗瘤作用的研究

Synergistic effects of IL-2 and IL-12 on antitumor activity of human PBMC in vitro

目的检测白细胞介素-2(IL-2)联合IL-12诱导人外周血单个核细胞(PBMC)体外抗肿瘤作用。方法分别应用MTT法和流式细胞仪法测定IL-2与IL-12协同诱导对体外培养的3种肿瘤细胞株的杀伤活性。结果IL-2与IL-12联合诱导的PBMC,对3株靶细胞的杀伤效应差异有显著性(P<0.05)。MTT法与流式细胞仪法两种方法检测的杀伤率有明显的相关性(r=0.997,P<0.05)。结论IL-2与IL-12协同对3种不同组织来源的实体瘤细胞株表现了较强的杀伤力,而杀伤率的差异与肿瘤细胞的不同免疫特征有关。

Objective To examine the in vitro antitumor activity of normal human peripheral blood mononuclear ceils （PBMC） induced by interleukin- 2 （IL- 2） and IL- 12. Methods MTr and flow cytometry assay were used to detect the cytotoxic effects of IL- 2 or/and IL - 12 on 3 cell lines. Results PBMC induced by IL- 2 and IL - 12 , had significantly different cytotoxic effects on three cell lines（ P 〈 0.05）. A good correlation was observed between the cytotoxicity by MTr and flow cytometry（r=0.997,P 〈0.05）. Conclusion The cytotoxic synergy by IL- 2 and IL-12 against 3 tumor cell lines in vitro is significantly differents. The different antitumor effects maybe associated with different immune characteristics of tumor cells.