摘要
参照GenBank中Purdue株全序列对5′和3′非编码区各设计一对特异性引物,经RT-PCR分别获得了2 957 bp和516 bp大小的片段,与预期结果大小相符。猪传染性胃肠炎病毒(TGEV)TS株与Puedue株、TH-98和FS772/70株的5′UTR核苷酸序列同源性分别为99.4%,99.3%,99.0%,TGEV TS株的3′UTR与Miller、Purdue、Niigata、h-5株的核苷酸同源性均为100.0%,与Aomori和Ogawa的同源性为99.3%。对非编码区的二级结构进行分析发现其具有复杂的二级结构。
Two UTR(untranslated region) of TS strain of Transmissible gastroenteritis virus(TGEV), 5' UTR and 3'UTR genes were obtained by RT-PCR using primers designed according to Purdue nucleotide sequence in the GenBank, and they are 2 957 bp and 516 bp. The homology at the nucleotide levels for the 5tUTR gene between TS and Purdur, TH-98, FS772/70 was found to be 99.40%, 99.3%, 99.0% respectively. The nucleotide homology of 3'UTR gene of TS shared 100.0% identity with that of strains Miller, Purdue, Niigata,h-5 and shared 99.3% with that of strains Aomori and Ogawa. The research results also indicate it has a complex secondary structure.
出处
《动物医学进展》
CSCD
2005年第12期71-74,共4页
Progress In Veterinary Medicine
基金
重大基础研究"973"前期专项(2004CCA00500)
关键词
猪传染性胃肠炎病毒
TS株
非编码区
克隆分析
Transmissible gastroenteritis virus
TS strain
untranslated region
clonging analysis