猪传染性胃肠炎病毒TS株非编码区的克隆及其一二级结构的分析

Clonging and Analysis of UTR of Transmissible gastroenteritis virus TS Strains

参照GenBank中Purdue株全序列对5′和3′非编码区各设计一对特异性引物,经RT-PCR分别获得了2 957 bp和516 bp大小的片段,与预期结果大小相符。猪传染性胃肠炎病毒(TGEV)TS株与Puedue株、TH-98和FS772/70株的5′UTR核苷酸序列同源性分别为99.4%,99.3%,99.0%,TGEV TS株的3′UTR与Miller、Purdue、Niigata、h-5株的核苷酸同源性均为100.0%,与Aomori和Ogawa的同源性为99.3%。对非编码区的二级结构进行分析发现其具有复杂的二级结构。

Two UTR（untranslated region） of TS strain of Transmissible gastroenteritis virus（TGEV）, 5＇ UTR and 3＇UTR genes were obtained by RT-PCR using primers designed according to Purdue nucleotide sequence in the GenBank, and they are 2 957 bp and 516 bp. The homology at the nucleotide levels for the 5tUTR gene between TS and Purdur, TH-98, FS772/70 was found to be 99.40%, 99.3%, 99.0% respectively. The nucleotide homology of 3＇UTR gene of TS shared 100.0% identity with that of strains Miller, Purdue, Niigata,h-5 and shared 99.3% with that of strains Aomori and Ogawa. The research results also indicate it has a complex secondary structure.