中药制剂对禽流感病毒H9N2、新城疫病毒、传染性支气管炎病毒和鸡毒支原体的攻毒保护试验

The Effect of Chinese Herb Medicine on the Protection of Chicken Challenged with AIV H9N2,NDV,IBV and Mycoplasma gallisepticum

采用以野菊花、穿心莲、柴胡等10味中药提取的中药制剂,连续给22日龄SPF鸡口服5 d后,以禽流感病毒H9N2、新城疫病毒F4、传染性支气管炎病毒强毒株M41和支原体S6强毒分别攻击,观察SPF鸡临床症状、发病率、病理学变化和分离病毒。结果表明,此中药制剂75 mg/只口服组,新城疫病毒、禽流感病毒和传染性支气管炎病毒攻击后发病率分别为12.5%,0%,10%;37.5 mg/只剂量口服组发病率分别为50%,10%,10%,且低剂量组病毒分离阳性。攻击支原体后,以上两个剂量组SPF鸡病变指数分别3.5和5.5,研究证实本中药制剂可以控制以上4种病原所致的家禽呼吸道病的发生。

The extract liquid which was composed of Yejuhua （Flos chrysanthemi）, Chuanxinlian （Herb Andrographitis）, Chaihu （Radix bupleuri） and the other Chinese herb medicines was applied continually to the 20-day-old specific pathogen free （SPF） chickens by oral drop. After five days of oral administration, the SPF chickens challenged with Mycoplasma galliseplicum （MG）, Newcastle disease virus （NDV）,Infectious bronchitis virus（IBV） and Avian influenza virus （AIV HgN2）,respectively,were monitored every day to determine the clinical signs, morbidity, lesion change and the virus isolation. The results demonstrated 12.5% ,0% ,10% of SPF chickens were suffered from challenging of vivid NDV,AIV and IBV in the medium dosage of 75 mg per chicken. Similarly, 50%, 10%, 10% of challenged chickens were unable to get protection in the lower dosage of 37.5 mg per chickens. The pathological index of infected chickens were 3.5 in medium dosage group and 5.5 in lower dosage group post inoculating with vivid MG. The above evidence showed that the extract herb medicine could be used to control the chicken respiratory disease.