缺口型试样管阵列进样系统与紫外检测毛细管电泳联用技术

Miniaturized Capillary Electrophoresis System with Slotted Micro-Vial Array Based Sample Introduction System and Ultraviolet Detection

将基于缺口型试样管阵列的微流控试样引入-系统与紫外检测-毛细管电泳系统联用,建立可实现自动化、高通量、连续试样引入的微型化毛细管电泳系统。试样引入系统由底部加工有缺口的试样管阵列构成,阵列固定于一维平移台上。实验时,通过平移试样管阵列,使毛细管和电极依次经缺口进入装有试样或缓冲液的试样管内,完成电动进样和电泳分离操作。该系统被用来快速分离复方新诺明片中的磺胺甲嗯唑(SMZ)和甲氧苄氨嘧啶(TMP),以考察系统分析性能。分析通量达到72样／h,试样间携出量为1．4％,对SMZ的分离塔板高度11μm。采用紫外检测对SMZ和TMP检出限(3σ)分别为9．8 mg／L和12．2 mg／L。

A high throughput sample introduction system that based on slotted micro-vial array coupled with a miniaturized capillary electrophoresis （CE） system with short capillary and ultraviolet detection to achieve automated and continuous sample introduction and separation was developed. The sample introduction system is composed of an array of horizonally positioned micro-sample vials with slits fabricated on the bottom of each vial. Sample introduction was performed by linearly moving the array of vials, allowing the capillary and electrode to sequentially enter the solutions in the vials through the slits .achieving sample introduction and separation operation. The present system was applied in fast separation of sulphamethoxazole （SMZ） and trimethoprim （TMP） in sulphatrim tablets, achieving a high throughput of 72/h and a low carryover of 1.4%. Separation efficiency of 11 μm plate height for SMZ was obtained. The detection limits（3σ）for SMZ and TMP were 9.8 mg/L and 12.2 mg/L, respectively.