摘要
分枝杆菌Mycobacteriumsp．M1可以有效降解大豆甾醇侧链得到雄甾4-烯-3,17-二酮 (AD)和雄甾-1,4-二烯-3,17-二酮(ADD)。通过该菌株细胞和细胞裂解液对底物AD的转化,表明了该菌株中存在甾酮C1．2位脱氢酶。利用酶活测定中活力高的细胞裂解液可以有效地降解大豆甾醇生成ADD。
The selective side-chain cleavage of stigmosterol to androsta-1 -diene-3,17-dione （AD） and androsta-1-diene-3,17-dione（ADD） by Mycobacterium sp. M1 was described. [ Distinct 3-ketoster- old-1-dehydrogenase activities in] Mycobacterium sp. M1 were found during the transformation of AD to ADD by the cells and cell extracts. According to the assay of 3-ketosteroid-l-dehydrogenase activity, the cell extracts of Mycobacterium sp. M1 containing high 3-ketosteroid-l-dehydrogenase activity were prepared which had the ability to convert stigmosterol to ADD effectively.
出处
《天津科技大学学报》
CAS
2005年第4期14-17,共4页
Journal of Tianjin University of Science & Technology
基金
天津市自然科学基金资助项目(043606911)
