转基因植物种植地土壤微生物区系生态变化及其外源基因的分子检测

Ecological changes of soil microorganism community after planting transgenic plant and molecular detect of alien target DNA

对转基因植物种植地土壤中微生物种群数量的影响及外源基因是否转移到土壤微生物中的可能性进行检测,是转基因植物生态安全评价的重要组成部分。本研究从云南省农业科学院大棚采集了转基因西红柿及对照土壤样品12份,采用平板稀释法对该土样中的微生物进行分离鉴定,结果表明种植转基因西红柿的土样中微生物的数量和种类有一定的变化。设计35s启动子、反义基因、卡那霉素抗性标记基因等特异引物对上述土样的总DNA,以及菌株DNA进行PCR扩增,均未检测到外源基因扩增产物。

Supervision of changes in microorganism species and quantity in soil after planting transgenie plants and possibility of alien target DNA transfered into soil microorganism was an important part of ecological security evaluation in transgenic plant. 12 transgenic tomato and contrast soil samples from Yunnan Academy of Agric. Sci. （YAAS） were collected and identified,and the microorganism were isolated by using flat dilution to determine the kinds and quantity. Results showed that there were some changes in quantity and species of microorganism in transgenie tomato soil samples, but no alien target DNA was detected from PCR products of total soil DNA and strain DNA amplified with specific primers of 35 s promoter, anti-Acc and NPTII gene.