三磷酸腺苷对巨噬细胞吞噬功能和微丝束重组的影响

EFFECTS OF ATP ON PHAGOCYTOSIS AND MICROFILAMENT BUNDLES REORGANIZATION OF MACROPHAGES

三磷酸腺苷（ＡＴＰ）对小白鼠腹腔巨噬细胞的吞噬功能有明显的促进作用，吞噬率由３７．２％±１１．７增加到７７．４％±１０．２３（Ｐ＜０．００５），吞噬指数由１．３１±０．３５增加到３．２０±０．５２（Ｐ＜０．０００５），有显著性差异。扫描电镜下观察到ＡＴＰ处理的小白鼠腹腔巨噬细胞多数都显示吞噬５～１０个鸡红细胞，并将其粘附在膜表面，而对照组的小白鼠腹腔巨噬细胞，只有少数显示吞噬和粘附２或３个鸡红细胞。用罗丹明－鬼笔环肽荧光探针标记和纽蛋白（ｖｉｎｃｕｌｉｎ）免疫荧光双重定位染色，观察到伴随巨噬细胞吞噬功能的加强，细胞内Ｆ－肌动蛋白小体消失，纽蛋白的点状荧光消失，微丝束出现，并沿细胞的纵轴排列，膜内表面的纽蛋白荧光斑（粘着斑）出现，并与微丝束相连接。表明ＡＴＰ促进巨噬细胞的吞噬功能，并与微丝束和纽蛋白组装改善紧密相关。

This experiment was carried out on mouse peritoneal macrophages(MΦ). Control group's mouse was injected intraperitoneally 0. 9 % NaCl/qd 0. 2ml. At the same time, experimental group's mouse was injected 0. 9 % NaCl/qd 0. 2ml(containing 4mg ATP). After 7 days,MΦ were then plated onto glass slide and cultured in incubator for 0. 5 hr at 37 ℃ . Almost all MΦ were adhered to substratum,they were used for further observation.Phagocytic rate and phagocytic index was obtained through counting of the Giemsa stained cell specimen. Cell Phagocytosis ultrastructural changes in MΦ of ATP-treated mouse were studied through scanning electron microscopy. Microfilament skeletal structures were studied by using double staining with fluorescence labelled phalloidin for F-actin and immunofluorescence of vinculin. It was found that.( 1 ) ATP dramatically increased phagocytic rate, from 37. 2 % ± 11 . 7 of control group to 77. 4 %± 10. 23 of ATP-group,an elevated phagocytic index from 1. 31 ± 0. 35 of control group to 3. 20±0. 56 of ATP-group . (2) ATP increased MΦ's phagocytosis and adhesion for checken red blood cells.(3) ATP improved microfilament bundles reorganization from F-actin dot-like structures of control group to more well-assembled stress fibers of ATP-group and vinculin adhesion plaques.It indicated that ATP promotion of phagocytic function was correlated closly with microfilament bundles and vinculin reorganization.