摘要
用造血祖细胞体外培养、造血生长因子检测和流式细胞术等方法,研究当归多糖(AP)诱导L-细胞(成纤维细胞株)产生造血生长因子及其对血细胞发生的调节作用。结果表明,L-细胞条件培养液(LcCM)对造血祖细胞(CFU-GM、BFU-E、CFU-E)的体外增殖呈双向调节作用;10%LcCM能促进骨髓造血细胞进入增殖活跃的S+M/G2期;经AP诱导制备的LcCM在有或无外源性造血生长因子(如Epo,BPA,GM-CSF)存在时均对造血祖细胞的克隆增殖显示较高刺激活性;AP或IL-1与造血生长因子间似无协同作用。本研究提示AP可能通过诱导造血微环境的成纤维细胞分泌某些造血生长因子,从而促进造血祖细胞增殖分化,这或许是当归"补血"的生物学机理之一。
Fibroblast is one of the major components in hematopoietic inductive microenvironment(HIM).By hematopoietic progenitor cell culture, hematopoietic growth factor(HGF) assay and flow-cytometry,HGF inducing by L-cell treated with Angelica Polysaccharide(AP) was studied. The results indicated that when without exogenous HGF, L-cell conditioned medium(LcCM) presented positive or negative effect on proliferation of BFU-E,CFU-E,CFU-GM. LcCM can actively promote bone marrow hematopoietic cell to enter the proliferative phase(S+M/G2 ) . AP LcCM , prepared by treating L-cell with AP, had much stronger stimulating activities on the proliferation of BFU-E, CFU-E and CFU-GM. There may be no synergic effects between HGF and AP or IL-1 in hematopoiesis. This research suggests that AP may enhance the hematopoiesis in mice by activating directly or indirectly the fibroblast in HIM and by promoting them to produce some HGFs(such as GM-CSF, Epo, BPA).These HGFs consequently regulate the hematopoiesis,This may be one of the mechanisms for the 'antianemic' effect of Angelica Sinensis.
出处
《解剖学报》
CAS
CSCD
北大核心
1996年第1期69-74,共6页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
当归多糖
造血生长因子
L-细胞
当归
中药药理学
Angelica polysaccharide
L-cell
Hematopoietic growth factor
Hematopoietic progenitor cell