微柱高效液相色谱法测定阿奇霉素含量

Study on Rapid Determination of Azithromycin by Solid Phase Extraction and Microcolumn High Performance Liquid Chromatography

研究了用固相萃取富集和预分离，微柱高效液相色谱快速测定阿奇霉素的方法；样品中的阿奇霉索用Waters Xterra^TMRP18反相固相萃取小柱预分离和富集，然后以Waters Xterra^TMRP18（1．0×50mm，2．5mm）微柱为固定相，四氢吡咯一醋酸缓冲溶液（pH11．0）和乙腈（50：50，v／v）为流动相分离，用二极管矩阵检测器检测，检测波长为215nm，样品中的阿奇霉素在5．0min内可达到基线分离．方法标准回收率为90％-101％，RSD为0．38％-2．8％．用该方法测定了注射液和尿样品中的阿奇霉索，结果令人满意．

A microcolumn high performance liquid chromatography （HPLC） method for the determination of azithromycin was studied. The azithromycin were enriched and separated by solid phase extraction with Waters Waters Xterra^TMRP18 cartridge. Then the azithromycin were separated on a Waters Xterra ^TMRp18 （1.0 × 50 mm, 2.5 mm） microcolumn with pyrrolidine - acetic acid buffer solution （ pH = 10.0） and acetonitrile （50： 50, v/v） as mobile phase at a flow - rate of 0.15 mL/min, and monitoring with photodiode array detector at 215 nm. The recoveries of the azithromycin are 90% - 101%, and the relative standard deviations are 0.38% - 2.8%. This method was applied to the determination of azithromycin in azithromycin aspartate for injection and human urine with good results.