黄芪注射液对大鼠肾缺血-再灌注损伤的影响

Effects of Astragalus Injection on Renal Ischemia Reperfusion Injury in Rats

目的探讨黄芪注射液对大鼠肾缺血-再灌注损伤的影响。方法建立肾缺血-再灌注损伤大鼠模型,25只大鼠随机分为治疗组(n=10)和模型组(n=15)。另设假手术组(n=5)作为对照。治疗组于肾动脉夹闭时给予黄芪注射液4 mL.kg-1,静脉输入,开夹再灌注时又给予原药量的1/2;模型组同时给予等量0.9%氯化钠溶液静脉输入。另取仅作麻醉、开腹、不阻断肾血流的5只大鼠作为对照组。观察肾组织中P-选择素、ICAM-1的表达以及丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性变化,检测血清尿素氮(BUN)和肌酐(Cr)的含量。结果缺血-再灌注后,模型组大鼠肾组织中P-选择素、细胞间粘附分子(ICAM-1)明显表达,MDA含量增高,SOD活性下降,BUN和Cr含量明显上升;治疗组经黄芪注射液处理后肾组织中P-选择素、ICAM-1表达受到抑制。相对模型组MDA含量降低,SOD活性增高,BUN和Cr含量降低。结论粘附分子P-选择素和ICAM-1参与肾缺血-再灌注损伤机制,黄芪注射液明显减少脂质过氧化反应,对肾缺血-再灌注损伤大鼠有保护作用。

Objective To investigate the effect and mechanism of astragalus injection on renal ischemia-reperfusion injury in rat. Methods Rat models of renal ischemia-reperfusion were established, and 25 male rats were randomly divided into 3 groups, treatment group with astragalus injection （n = 10）, renal ischemia-reperfusion injury model group （n -- 15 ） and sham operation group （n -- 5 ） as the control . Astragalus injection （4 mL·kg^-1） was given intravenously during clamping renal artery in treatment group and one half of the dose was infused after opening the clamp during reperfusion. In model group equal amount of 0.9% sodium chloride solution was given. In the sham operation group （control group）, the rats were anesthetized with laparotomy but without blocking renal blood flow. The P-selectin and ICAM-1 expression, malonyldialdehyde （MDA） contents and super oxide dismutase（ SOD ） activities in renal tissues, level of serum blood urea nitrogen （BUN） and creatinine （ Cr ） were detected. Results After renal ischemia reperfusion injury, there were significantly up-regulated expressions of P-selectin and ICAM-1, with increased MDA content and declined SOD activity in renal tissue, and significant increase in BUN and Cr content in model group, and in treatment group with astragalus injection these effects were significantly depressed as compared with model group. Conclusion It is thought that the adhesion molecules P-selectin and ICAM-1 take part in the process of renal ischemia-reperfusion injury, and astragalus injection significantly reduce the reaction of lipid peroxidation with a protective effect on renal ischemia-reperfusion iniury .