促进马扎德甜樱桃砧木组培苗生根的研究

Techniques for encouraging rooting of tissue-cultured shoots in sweet cherry on rootstocks 'Mazzard'

以甜樱桃砧木马扎德（Mazzard）的组培苗为试材，研究促进其生根的方法。结果表明，最佳生根培养基为1／2MS＋0．3mg／LIBA。将分化组培苗转入该培养基，移至温室中黑暗处理10天后，在1500～40001x自然光下培养，生根率可达93．5％。对瓶内未生根的幼苗，用1000mg／LIBA浸泡60秒后扦插到温室育苗盘中，1个月后生根率可达80％以上。

Trials with wild ‘Mazzard＇ sweet cherry tissue - cultured shoots manifested that the optimal medium for rooting was 1/2MS ＋ 0.3 mg/L IBA. The rooting rate of 93.5% was obtained by the following practice：the differentiated tissue - cultured shoots were transplanted onto the above - mentioned medium and dark - cultured in a greenhouse for ten days, then subjected to 1500 - 40001x of natural light. There was got an additional rooting rate over 80% when the unrooted shoots were immersed in 1000mg/L IBA solution for 60s, then transplanted to nursery trays to be cultured in a greenhouse after one month.