毛细管电泳法同时测定血浆中的美西律、利多卡因和布比卡因的浓度

Simultaneous determination of mexiletine,lidovacaine and bupivacaine in plasma by capillary electrophoresis

采用毛细管电泳法同时测定血浆中美西律、利多卡因和布比卡因的浓度.取0.5 mL血浆,用乙醚提取后吹干,重组后进样于毛细管电泳仪进行分离测定.电泳条件:分离用缓冲液为75 mmol/L NaH2PO4溶液(pH 3.0),温度30℃,运行电压26 kV,紫外检测,波长200nm,压力进样5 s.本法在0.1～4.0μg/mL范围内线性关系和精密度良好,方法回收率在96%～105%之间,检出限均为0.02μg/mL,可满足临床监测需要.

An electrophoretic method was established for the determination of mexiletine, lidovacaine and bupivacaine in plasma. The plasma was extracted by ether, and the extract was evaporated to dryness. The residue was reconstituted and injected for analysis. The electrophoresis conditions were as follows： running buffer 75 mmol/L NaH2PO4 （pH 3.0）, temperature 30℃, voltage 26 kV, UV detection at 200 nm, pressure injection for 5 s. The established method has good linearity and precision in the range of 0.1 -4.0 μg/mL, which is suitable for routine monitoring.