小鼠异种胰岛移植排斥反应研究

The rejection of xenoislet transplantation

目的观察小鼠对肾被膜下异种胰岛移植物的排斥反应规律和免疫病理学特点。方法将大鼠胰岛移植Nd,鼠肾被膜下，分别于术后当日、第1、2、4、5、6天取出移植物，研究异种胰岛排斥的病理组织学形态特点和过程。结果异种胰岛移植可有功能存活（5．9±1．2）d，在自然情况下，胰岛在术后6d左右完全被排斥，术后4d胰岛形态已不完整，单核细胞浸润明显增多。移植物附近未发现IgG＋IgM的免疫沉积。结论异种胰岛的排斥反应是一个以单核细胞浸润为特征的渐进性过程，移植后4～6d达排斥高峰，CD4^＋T细胞可能在急性细胞性排斥反应中起重要作用，而体液免疫没有或很少参与。

Objective To-find the process of rejection and histological characteristics of xenografts by transplanting islets of rats into the kidney subcapsule of the mice. Methods The islets of Langerhans were transplanted under the kidney capsule of diabetic mouse. The implants were removed at the day of transplantation, the day 1,2, 4, 5, 6 after transplantation, respectively. The histopathological characteristics of xenograft rejection were studied. Results Xenotransplantation could maintain euglycemic for （5.9 ± 1.2） days. The majority of islets grafted under kidney capsule were rejected completely within 6 days after transplantation without the administration of immunosuppressive agents. On the day 4, the islets were morphologically damaged and mononuclear ceUs infiltration increased massively. No deposits of IgG and IgM were seen adjacent or within the xenografts. Conclusion The xenograft rejection is a progressing process characterized by mononuclear cellular infiltration that reaches the peak between 4-6 days after transplantation. CD4^＋ T cells might play an important role in rejection of the islet xenografts. However, humoral rejection is not or little involved in this reaction.