摘要
作者报告了应用Ｆｕｒａ－２单细胞显微荧光探测技术观察活血去瘀中药丹参注射液对肝缺血再灌注期肝细胞内游离钙浓度［（Ｃａ２＋）ｉ］影响的实验及临床研究，同期观察肝组织脂质过氧化自由基ＲＯＯ·，电子自旋共振信号幅值（ＥＳＲ技术）和肝细胞超微结构病理改变，结果发现丹参可明显降低肝细胞内（Ｃａ２＋）ｉ，ＲＯＯ·幅值，减轻细胞损伤的病理程度。
InrecentyearstherehasbeenconsiderableinterestconcerningtheroleofhepatocelularCa2+overloadwhichprobablywasamajorfactorinhepaticischemia/reperfusioninjury.Westudiedtheefectofradixsalviaemiltiorrhizae(RSM)oncytosolicfreecalciumionconcentration[(Ca2+)i](nM)inisolatedhepatocytesinratsandpatientswithischemiareperfusionbymicroflurometryusingfluorescent(Ca2+)iindicatorFura-2/AM.Changesoflipidperoxidefreeradical(ROO·)signalrangeswithinthelivertissuebyESRtechniqueandthoseofhepatocelularultrastructurebyelectronmicroscopewerealsoobserved.TheresultsshowedthatRSMreducedlevelsof(Ca2+)iandROO·Ymax(mm)ESRsignalrangs.RSMhadanefectonprotectinghepatocytesagainstischemia/reperfusioninjuryasausefulre-ceptor-operatedcalciumchannels(ROC)blocker.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1996年第2期98-101,共4页
Chinese Journal of Surgery
